Inui J, Miura Y, Imamura H
Nihon Yakurigaku Zasshi. 1977 Mar;73(2):143-50.
A system of digital measuring of the cardiac action potential was constructed in order to measure quickly and accurately the resting potential or maximum diastolic potential, overshoot potential, amplitude, time for 50% repolarization and time for 90% repolarization. This system consists of a dual-beam oscilloscope, a digital voltage meter, a digital interval meter and a simple circuit constructed by operational amplifiers. The measurement of the action potential was achieved by tracing only the resting potential or the maximum diastolic potential and the crest of the action potential on the oscilloscope using another beam. The time for measurement is usually 10-15 sec and errors in measurement were considered to be negligible. This system is considered to be useful for digital measurement of the action potential of various cardiac tissues. This system was applied for observation of effects of bufetolol, an adrenergic beta-receptor blocking drug on the action potential of the dog Purkinje fibres. Bufetolol (10(-5) M) caused a slight depolarization of the maximum diastolic potential, decreased overshoot potential, amplitude and maximum rate of rise, and shortened the time for 50% repolarization of the action potential. Bufetolol (10(-4) M) additionally prolonged the time for 90% repolarization.
为了快速、准确地测量静息电位或最大舒张电位、超射电位、振幅、50%复极化时间和90%复极化时间，构建了一个心脏动作电位数字测量系统。该系统由双光束示波器、数字电压表、数字间隔计和由运算放大器构成的简单电路组成。通过使用另一束光束在示波器上仅追踪静息电位或最大舒张电位以及动作电位的波峰来实现动作电位的测量。测量时间通常为10 - 15秒，测量误差可忽略不计。该系统被认为对各种心脏组织动作电位的数字测量有用。该系统应用于观察肾上腺素能β受体阻断药布非洛尔对犬浦肯野纤维动作电位的影响。布非洛尔（10(-5)M）使最大舒张电位略有去极化，降低了超射电位、振幅和最大上升速率，并缩短了动作电位50%复极化的时间。布非洛尔（10(-4)M）还延长了90%复极化的时间。
