Pinet Florence, Beseme Olivia, Cieniewski-Bernard Caroline, Drobecq Hervé, Jourdain Sabine, Lamblin Nicolas, Amouyel Philippe, Bauters Christophe
INSERM U744, Lille, France.
Proteomics. 2008 May;8(9):1798-808. doi: 10.1002/pmic.200700781.
Recent improvements in therapeutic strategies did not prevent left ventricular remodeling (LVR), which remains a common event (30%) after acute myocardial infarction (AMI). We report the use of a systematic approach, based on comparative proteomics, to select circulating biomarkers that may be associated with LVR. We selected 93 patients enrolled in a prospective study. These patients with anterior wall Q-wave AMI underwent echocardiographic follow-up at hospitalization, 3 months and 1 year after AMI. They were divided into three groups (no, low, or high remodeling). Plasma samples of these patients (day 5 of hospitalization) were processed and stored at -80 degrees C within 2 h and analyzed using SELDI-TOF protein chip technology. This systematic approach allowed to select candidate proteins modulated by LVR: post-translational variants of alpha1-chain of haptoglobin (Hpalpha1) corresponding to m/z 9493, 9565, and 9623, which were more elevated in remodeling patients. The peak 9493 m/z was shown having a receiving-operating characteristic (ROC) value of 0.71 between non- and remodeling patients. SELDI-TOF approach may lead to the identification of circulating proteins associated with LVR. Whether these candidate proteins will help to identify patients who are at high risk of heart failure after AMI will have to be tested in future studies.
近期治疗策略的改进并未阻止左心室重构（LVR），在急性心肌梗死（AMI）后，LVR仍然是常见事件（发生率为30%）。我们报告了一种基于比较蛋白质组学的系统方法，用于筛选可能与LVR相关的循环生物标志物。我们选择了93例纳入前瞻性研究的患者。这些前壁Q波AMI患者在住院时、AMI后3个月和1年接受了超声心动图随访。他们被分为三组（无重构、轻度重构或重度重构）。这些患者（住院第5天）的血浆样本在2小时内进行处理并储存在-80℃，然后使用表面增强激光解吸电离飞行时间（SELDI-TOF）蛋白质芯片技术进行分析。这种系统方法能够筛选出受LVR调节的候选蛋白：对应于质荷比9493、9565和9623的触珠蛋白α1链（Hpα1）的翻译后变体，在重构患者中升高更为明显。质荷比9493的峰在非重构患者和重构患者之间的受试者工作特征（ROC）值为0.71。SELDI-TOF方法可能会导致鉴定出与LVR相关的循环蛋白。这些候选蛋白是否有助于识别AMI后心力衰竭高危患者，有待未来研究进行验证。
