Cowan David A
Drug Control Centre, Department of Forensic Science and Drug Monitoring, Pharmaceutical Science Division, King's College London, 150 Stamford Street, London SE1 9NH, UK.
Essays Biochem. 2008;44:139-48. doi: 10.1042/BSE0440139.
The analysis of sports samples for prohibited substances began in the 1960s and has developed since then using modern technologies close to the latest scientific discoveries. In this chapter the latest techniques and applications are described as well as the role of the World Anti-Doping Agency as the controlling body for the implementation of these tests. For small molecules, apart from the routine use of GC-MS, the newer techniques include the use of isotope ratio MS to detect testosterone and nandrolone administration and LC-MS/MS (liquid chromatography-tandem MS) to detect diuretics. For large molecules, several applications of LC-MS/MS are described as well as immunoprocedures for erythropoietin and human growth hormone. Finally, the latest method to detect homologous blood transfusion is briefly described.
对体育样本进行违禁物质分析始于20世纪60年代，此后借助接近最新科学发现的现代技术不断发展。本章介绍了最新技术及其应用，以及世界反兴奋剂机构作为实施这些检测的监管机构所发挥的作用。对于小分子物质，除了常规使用气相色谱 - 质谱联用仪（GC-MS）外，新技术还包括使用同位素比质谱仪检测睾酮和诺龙的使用情况，以及使用液相色谱 - 串联质谱仪（LC-MS/MS）检测利尿剂。对于大分子物质，介绍了液相色谱 - 串联质谱仪的几种应用以及促红细胞生成素和人生长激素的免疫检测方法。最后，简要描述了检测同源输血的最新方法。
