Tawara Akihiko, Tou Norihiko, Kubota Toshiaki, Harada Yukinori, Yokota Kiyomi
Department of Ophthalmology, University of Occupational & Environmental Health, Japan, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Graefes Arch Clin Exp Ophthalmol. 2008 Jul;246(7):1021-8. doi: 10.1007/s00417-008-0800-0. Epub 2008 Apr 2.
To immunohistochemically examine the localization of the extracellular matrix (ECM) in the trabecular meshwork (TM) in steroid-induced glaucoma (SG) specimens.
We morphologically and immunohistochemically examined six trabecular tissues from three eyes with SG, two with primary open angle glaucoma (POAG), and one without glaucoma. For the morphological study, the ultra-microtome sections were observed using an electron microscope. For the light microscopic immunohistochemical analyses, frozen sections were stained by the avidin-biotin complex method using anti-type IV collagen, anti-type VI collagen, anti-heparan sulfate proteoglycan (HSPG), anti-fibronectin or anti-myocilin (MYOC) antibody.
The morphological examinations revealed accumulations of basement membrane-like and fine fibrillar-like materials in the outer TM of SG specimen. Type IV collagen, HSPG and fibronectin antibodies in SG specimens showed a greater degree of staining in the outer TM in comparison to the POAG and non-glaucomatous specimens; in contrast, the other antibodies including the type VI collagen and MYOC, did not.
The localization of ECMs in the TM is different in SG in comparison to that in POAG patients.
通过免疫组织化学方法检测类固醇性青光眼（SG）标本中小梁网（TM）细胞外基质（ECM）的定位。
我们对来自3只SG眼、2只原发性开角型青光眼（POAG）眼和1只非青光眼眼的6个小梁组织进行了形态学和免疫组织化学检查。形态学研究中，使用电子显微镜观察超薄切片。光镜免疫组织化学分析中，冰冻切片采用抗IV型胶原、抗VI型胶原、抗硫酸乙酰肝素蛋白聚糖（HSPG）、抗纤连蛋白或抗肌纤蛋白（MYOC）抗体，通过抗生物素蛋白-生物素复合物法进行染色。
形态学检查显示SG标本的外TM中有基底膜样和细纤维样物质积聚。与POAG和非青光眼标本相比，SG标本中的IV型胶原、HSPG和纤连蛋白抗体在外TM中的染色程度更高；相比之下，包括VI型胶原和MYOC在内的其他抗体则不然。
与POAG患者相比，SG患者TM中ECM的定位有所不同。
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