Allegrezza-Giulietti A, Serretti R, Beccerica E, Muti S, Ferretti G, Cervini C
Institute of Rheumatology, Faculty of Medicine, University of Ancona, Italy.
J Cell Biochem. 1991 Nov;47(3):242-50. doi: 10.1002/jcb.240470310.
The aim of this research was to evaluate in vitro interactions between platelets and polymorphonuclear leukocytes. The effects of supernatant from thrombin-activated platelets and two platelet release products (adenosine triphosphate and beta-thromboglobulin) were tested on the following features of polymorphonuclear leukocytes activation: opsonized zymosan and phorbol myristate acetate stimulated chemiluminescence, release of membrane bound calcium, NADPH-oxidase activity, and membrane fluidity (fluorescent polarization). The results showed that the addition of platelet supernatant to polymorphonuclear leukocytes induces a significant activation of cells. On the other hand, after three hours of preincubation of polymorphonuclear leukocytes with platelet supernatant, a decreased response of polymorphonuclear leukocytes to stimulation with phorbol myristate acetate, a significant decrease in NADPH-oxidase activity, and a lowered membrane fluidity were observed. Adenosine triphosphate modulated only opsonized zymosan stimulated chemiluminescence, with and without preincubation with polymorphonuclear leukocytes. Beta-thromboglobulin caused a decrease of the chemiluminescent response of polymorphonuclear leukocytes, using both agonists, with and without preincubation with polymorphonuclear leukocytes. Moreover beta-thromboglobulin only caused a decrease of the polymorphonuclear leukocytes membrane fluidity without preincubation with the cells. These results support the thesis that platelets have a "time-related" modulating activity on polymorphonuclear leukocytes.
本研究的目的是评估血小板与多形核白细胞之间的体外相互作用。测试了凝血酶激活血小板的上清液以及两种血小板释放产物（三磷酸腺苷和β-血小板球蛋白）对多形核白细胞激活的以下特征的影响：经调理的酵母聚糖和佛波酯肉豆蔻酸酯刺激的化学发光、膜结合钙的释放、NADPH氧化酶活性以及膜流动性（荧光偏振）。结果表明，向多形核白细胞中添加血小板上清液可诱导细胞的显著激活。另一方面，多形核白细胞与血小板上清液预孵育三小时后，观察到多形核白细胞对佛波酯肉豆蔻酸酯刺激的反应降低、NADPH氧化酶活性显著降低以及膜流动性降低。无论是否与多形核白细胞预孵育，三磷酸腺苷仅调节经调理的酵母聚糖刺激的化学发光。使用两种激动剂，无论是否与多形核白细胞预孵育，β-血小板球蛋白都会导致多形核白细胞化学发光反应降低。此外，β-血小板球蛋白仅在未与细胞预孵育的情况下导致多形核白细胞膜流动性降低。这些结果支持了血小板对多形核白细胞具有“时间相关”调节活性这一论点。
