Colonno R J, Banerjee A K
Cell. 1976 Jun;8(2):197-204. doi: 10.1016/0092-8674(76)90003-9.
Purified virions of vesicular stomatitis virus (VSV) are capable of synthesizing two distinct types of virus-specific RNA in vitro. The first consists of several viral mRNAs which have been previously shown to contain the blocked 5' terminal sequence GpppApApCpApGp and 3' terminal poly(A). The second type of RNA has an unblocked 5' terminus and does not contain poly(A) stretches long enough to bind to oligo (dT)-cellulose columns. It migrates in 20% polyacrylamide gels as a single homogeneous peak with an estimated chain length of 68 nucleotides. Base analysis demonstrated that this small RNA molecule is composed of 48% AMP, 20% CMP, 11% GMP, and 21% UMP. The 5' terminal sequence of the small RNA is ppApCpGp, which appears to be complementary to the 3' terminal sequence of the VSV genome RNA (...PypGpU). These results indicate that this small RNA molecule probably represents the intitiated lead-in RNA segment which is removed during formation of VSV mRNAs by a possible processing mechanism.
水泡性口炎病毒（VSV）的纯化病毒粒子能够在体外合成两种不同类型的病毒特异性RNA。第一种由几种病毒mRNA组成，先前已证明它们含有封闭的5'末端序列GpppApApCpApGp和3'末端多聚（A）。第二种RNA的5'末端未封闭，且不含有足够长的多聚（A）片段以结合到寡聚（dT）-纤维素柱上。它在20%聚丙烯酰胺凝胶中迁移时呈现为单一的均匀峰，估计链长为68个核苷酸。碱基分析表明，这种小RNA分子由48%的AMP、20%的CMP、11%的GMP和21%的UMP组成。小RNA的5'末端序列是ppApCpGp，它似乎与VSV基因组RNA的3'末端序列（...PypGpU）互补。这些结果表明，这种小RNA分子可能代表了在VSV mRNA形成过程中通过一种可能的加工机制被去除的起始导入RNA片段。
