水泡性口炎病毒体内mRNA中的甲基化和封闭5'末端

Methylated and blocked 5' termini in vesicular stomatitis virus in vivo mRNAs.

作者信息

Moyer S A, Abraham G, Adler R, Banerjee A K

出版信息

Cell. 1975 May;5(1):59-67. doi: 10.1016/0092-8674(75)90092-6.

Abstract

Methyl groups derived from 3H-methyl methionine were incorporated into vesicular stomatitis virus (VSV) MRNAs isolated from infected cells. Sequential degradation of the 12-18S viral mRNA species with ribonuclease T2, penicillium nuclease, and alkaline phosphatase yielded a single 3H-labeled dinucleotide. A similar resistant 32P-labeled fragment was obtained by digesting VSV mRNA uniformly labeled with 32P. This methylated and blocked oligomer was further cleaved with nucleotide pyrophosphatase, yielding two methylated 5' nucleotides. We postulate that the 5' terminal structure of the vivo 12-18S VSV mRNA contains 7-methylguanosine linked by a 5'-5' pyrophosphate bond to a methylated derivative of adenosine. In contrast to the mRNAs (+ strand), the VSV genome RNA ( MINUS STRAND) IS NOT BLOCKED.

摘要

源自3H-甲基甲硫氨酸的甲基基团被掺入从感染细胞中分离出的水疱性口炎病毒（VSV）mRNA中。用核糖核酸酶T2、青霉核酸酶和碱性磷酸酶对12 - 18S病毒mRNA进行顺序降解，产生了一个单一的3H标记二核苷酸。通过消化用32P均匀标记的VSV mRNA获得了类似的抗性32P标记片段。这个甲基化且封闭的寡聚物用核苷酸焦磷酸酶进一步切割，产生了两个甲基化的5'核苷酸。我们推测，体内12 - 18S VSV mRNA的5'末端结构包含通过5'-5'焦磷酸键与腺苷的甲基化衍生物相连的7-甲基鸟苷。与mRNA（正链）不同，VSV基因组RNA（负链）没有被封闭。