Hu Xiaoping, Liu Xin, Tani Akio, Kimbara Kazuhide, Kawai Fusako
College of Plant Protection, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, 712100, P R China.
Biosci Biotechnol Biochem. 2008 Apr;72(4):1115-8. doi: 10.1271/bbb.70749. Epub 2008 Apr 7.
Polypropylene glycol (PPG)-assimilating Sphingobium sp. strain PW-1 was grown on 0.5% PPG 700. PPG and its metabolites were analyzed by MALDI-TOF mass spectrometry. An oxidized form of a terminal alcohol group appeared with each molecular species as a metabolite. Cell-free extract showed PPG dehydrogenase activity. In this way, the oxidative metabolic pathway for PPG was confirmed.
能够同化聚丙二醇(PPG)的鞘氨醇单胞菌属(Sphingobium sp.)菌株PW-1在0.5%的PPG 700上生长。通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF质谱)对PPG及其代谢产物进行了分析。每种分子种类的代谢产物中都出现了末端醇基的氧化形式。无细胞提取物显示出PPG脱氢酶活性。通过这种方式,证实了PPG的氧化代谢途径。