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一株聚丙二醇降解菌——微小杆菌属7号菌株的全基因组序列

Complete Genome Sequence of a Polypropylene Glycol-Degrading Strain, Microbacterium sp. No. 7.

作者信息

Ohtsubo Yoshiyuki, Nagata Yuji, Numata Mitsuru, Tsuchikane Kieko, Hosoyama Akira, Yamazoe Atsushi, Tsuda Masataka, Fujita Nobuyuki, Kawai Fusako

机构信息

Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan

Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.

出版信息

Genome Announc. 2015 Dec 10;3(6):e01400-15. doi: 10.1128/genomeA.01400-15.

Abstract

Microbacterium (formerly Corynebacterium) sp. No. 7 was isolated from activated sludge as a polypropylene glycol (PPG)-assimilating bacterial strain. Its oxidative PPG degradation has been proposed on the basis of PPG dehydrogenase activity and the metabolic products. Here, we report the complete genome sequence of Microbacterium sp. No. 7. The genome of the strain No. 7 is composed of a 4,599,046-bp circular chromosome and two linear plasmids. The whole finishing was conducted in silico with aids of the computational tools GenoFinisher and AceFileViewer. Strain No. 7 is available from the Biological Resource Center, National Institute of Technology and Evaluation (NITE) (Tokyo, Japan).

摘要

微杆菌属(以前的棒杆菌属)菌株7是从活性污泥中分离出来的一株能同化聚丙二醇(PPG)的细菌。基于PPG脱氢酶活性和代谢产物,已提出其对PPG的氧化降解作用。在此,我们报告微杆菌属菌株7的完整基因组序列。菌株7的基因组由一条4,599,046 bp的环状染色体和两个线性质粒组成。借助计算工具GenoFinisher和AceFileViewer在计算机上完成了整个基因组的组装。菌株7可从日本国立技术与评价研究所(NITE)生物资源中心(东京)获得。

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本文引用的文献

1
Complete genome sequence of Acidovorax sp. strain KKS102, a polychlorinated-biphenyl degrader.
J Bacteriol. 2012 Dec;194(24):6970-1. doi: 10.1128/JB.01848-12.
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GenomeMatcher: a graphical user interface for DNA sequence comparison.
BMC Bioinformatics. 2008 Sep 16;9:376. doi: 10.1186/1471-2105-9-376.
4
Proposed oxidative metabolic pathway for polypropylene glycol in Sphingobium sp. strain PW-1.
Biosci Biotechnol Biochem. 2008 Apr;72(4):1115-8. doi: 10.1271/bbb.70749. Epub 2008 Apr 7.
5
Isolation of bacteria able to grow on both polyethylene glycol (PEG) and polypropylene glycol (PPG) and their PEG/PPG dehydrogenases.
Appl Microbiol Biotechnol. 2007 Jan;73(6):1407-13. doi: 10.1007/s00253-006-0616-y. Epub 2006 Oct 17.

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