Ralene L A, Dumchius A S, Stropus R A, Butkus R Iu, Shal'chius K M
Arkh Anat Gistol Embriol. 1991 May;100(5):73-8.
By means of histological and histochemical methods slices of biopsies of the canine musculus latissimus dorsi have been investigated after electroneurostimulation for three months through the thoracodorsal nerve in situ and after cutting its initial part. Frequency of contractions increases gradually from 30 up to 80 per 1 min every 2 weeks. The preparations are stained with hematoxylin--eosin. Histochemical reactions for adenosinetriphosphatase (ATPase) (incubational medium pH 4.3 and 10.3) and for acetylcholinesterase (AChE) are performed. The muscle, stimulated in situ, preserves its normal structure. Transformation of muscle contractions from fast to slow, as well as increasing AChE activity in myoneural synapses are revealed. The muscle, stimulated after its cutting at its beginning, is subjected to adipose dystrophy. Activity of ATPase and AChE do not differ from that in the control.
通过组织学和组织化学方法,对犬背阔肌活检切片进行了研究。在原位通过胸背神经进行电神经刺激3个月后,以及切断其起始部分后,观察这些切片。收缩频率每2周从每分钟30次逐渐增加到80次。标本用苏木精-伊红染色。进行了三磷酸腺苷酶(ATPase)(孵育介质pH 4.3和10.3)和乙酰胆碱酯酶(AChE)的组织化学反应。在原位受刺激的肌肉保持其正常结构。发现肌肉收缩从快速向慢速转变,以及肌神经突触中AChE活性增加。在其起始处切断后受刺激的肌肉发生脂肪性营养不良。ATPase和AChE的活性与对照组无差异。