[肿瘤患者外周血单个核细胞中LAIR-1的表达上调]
[The up-regulated expression of LAIR-1 in tumor patients PBMC].
作者信息
Xue Jiang-nan, Wang Chun-yan, Zhang Xiao-shu, Cao Qi-zhi, Su Zhen-guo
机构信息
Department of Immunology, Binzhou Medical University, Yantai 264003, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Apr;24(4):373-4, 377.
AIM
To investigate the expression of LAIR-1 in patients with tumors and the function of LAIR-1 in anticancer immunity.
METHODS
A sandwich ELISA was employed to detect the serum sLAIR-1 from tumor patients and healthy individuals. The expression of LAIR-1 in CD4+ T cells, CD8+ T cells, NK cells and B cells isolated from the peripheral blood of cancer patients was examined by fluorescence staining and flow cytometry.
RESULTS
The level of serum sLAIR-1 in tumor patients was higher than that in healthy individuals [4.6+/-3.2 microg/L vs 3.9+/-3.0 microg/L, P<0.05]. NK cells, CD4+ T cells and CD8+ T cells expressed the up-regulated LAIR-1. The ratio of CD4/CD8 in lung cancer patients decreased significantly while the percentage of B cells in cancer patients increased greatly compared with healthy individuals.
CONCLUSION
The expression of LAIR-1 is up-regulated in tumor patients, which may contribute to cancer immune escape.
目的
研究LAIR-1在肿瘤患者中的表达情况以及LAIR-1在抗癌免疫中的功能。
方法
采用夹心ELISA法检测肿瘤患者和健康个体血清中的可溶性LAIR-1（sLAIR-1）。通过荧光染色和流式细胞术检测从癌症患者外周血中分离出的CD4⁺ T细胞、CD8⁺ T细胞、自然杀伤细胞（NK细胞）和B细胞中LAIR-1的表达。
结果
肿瘤患者血清sLAIR-1水平高于健康个体[4.6±3.2μg/L对3.9±3.0μg/L，P<0.05]。NK细胞、CD4⁺ T细胞和CD8⁺ T细胞表达上调的LAIR-1。与健康个体相比，肺癌患者的CD4/CD8比值显著降低，而癌症患者中B细胞的百分比大幅增加。
结论
肿瘤患者中LAIR-1的表达上调，这可能有助于癌症免疫逃逸。
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