壳聚糖与血红蛋白之间的相互作用行为。
Interaction behaviors between chitosan and hemoglobin.
作者信息
Chen Liuhua, Tianqing Liu
机构信息
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jinagsu, 25002, PR China.
出版信息
Int J Biol Macromol. 2008 Jun 1;42(5):441-6. doi: 10.1016/j.ijbiomac.2008.02.005. Epub 2008 Mar 4.
The interaction and association between chitosan and hemoglobin (Hb) are studied by UV-vis and fluorescence spectroscopies, viscometry, circular dichroism, dynamic and static light scattering. Chitosan can obviously associate with Hb to form protein-chitosan complexes, which affects microstructure of Hb. The distance between the first association site of chitosan with 214-tryptophan residue in Hb is about 5.473 nm. The intrinsic UV-vis absorption and fluorescence intensities of Hb increase with an increase of chitosan concentration. The alpha-helix in Hb is drawn and changed into beta-sheet.
通过紫外可见光谱、荧光光谱、粘度测定、圆二色性、动态和静态光散射研究了壳聚糖与血红蛋白（Hb）之间的相互作用和缔合。壳聚糖能明显与Hb缔合形成蛋白质-壳聚糖复合物，这会影响Hb的微观结构。壳聚糖与Hb中214-色氨酸残基的第一个缔合位点之间的距离约为5.473nm。Hb的固有紫外可见吸收和荧光强度随壳聚糖浓度的增加而增加。Hb中的α-螺旋被拉伸并转变为β-折叠。
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