Bredenbeck Anne, Hollstein Verena M, Trefzer Uwe, Sterry Wolfram, Walden Peter, Losch Florian O
Clinical Research Group Tumor Immunology, Department of Dermatology, Skin Cancer Center Charité, Charité-Universitätsmedizin Berlin, 10098 Berlin, Germany.
Gene. 2008 May 31;415(1-2):68-73. doi: 10.1016/j.gene.2008.02.019. Epub 2008 Feb 29.
Cancer/testis antigens (CTA) are expressed in cancers and testis or placenta only and, therefore are considered promising targets for cancer immunotherapy and diagnosis. One family of CTA is the MAGEA family which comprises 13 members and was shown to be expressed synchronously with members from the CSAG (TRAG-3) family of CTA. The MAGEA genes are arranged in 4 subclusters located on the X chromosome. Subcluster III exposes a remarkable gene organization with an inverted repeat (IR) DNA structure of a triplicated couplet of a MAGEA gene and a CSAG gene. Analyzing the mRNA expression pattern of all genes of the MAGEA and CSAG family of cancer/testis genes, we show that the MAGEA and CSAG genes encoded in the large IR are expressed coordinately and independent from the MAGEAs encoded outside the IR. These results reinforce our hypothesis that the large MAGEA/CSAG-IR DNA structure has an impact on the regulation of gene expression.
癌胚抗原（CTA）仅在癌症、睾丸或胎盘中表达，因此被认为是癌症免疫治疗和诊断的有前景的靶点。CTA的一个家族是MAGEA家族，它由13个成员组成，并且已显示与CTA的CSAG（TRAG-3）家族的成员同步表达。MAGEA基因排列在位于X染色体上的4个亚簇中。亚簇III呈现出显著的基因组织，具有一个由MAGEA基因和CSAG基因的三联体重复组成的反向重复（IR）DNA结构。通过分析癌胚基因的MAGEA和CSAG家族所有基因的mRNA表达模式，我们发现大IR中编码的MAGEA和CSAG基因是协同表达的，且独立于IR外编码的MAGEA基因。这些结果强化了我们的假设，即大的MAGEA/CSAG-IR DNA结构对基因表达调控有影响。
