一种用于从染色的十二烷基硫酸钠-聚丙烯酰胺凝胶中回收蛋白质的纸质电洗脱系统。

A paper-based electroelution system for protein recovery from stained sodium dodecyl sulfate-polyacrylamide gels.

作者信息

Branco Alan T, Ferreira Beatriz dos S, de Souza Filho Gonçalo A

机构信息

Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque Califórnia, 28015-603 Campos dos Goytacazes, RJ, Brazil.

出版信息

Anal Biochem. 2008 Oct 15;381(2):267-9. doi: 10.1016/j.ab.2008.03.025. Epub 2008 Mar 22.

DOI:10.1016/j.ab.2008.03.025

PMID:18396142

Abstract

Electroelution is a widely used methodology for protein purification. In this study, a practical and low-cost system for protein electroelution from stained polyacrylamide gels was developed. For this, a horizontal protein electroelution cuve was constructed with glass plates, 1.5-ml capacity microcentrifuge tubes, and dialysis membrane. Analyses of the system efficiency showed high protein recovery from nonfixed and fixed sodium dodecyl sulfate (SDS)-polyacrylamide gels.

摘要

电洗脱是一种广泛应用于蛋白质纯化的方法。在本研究中，开发了一种用于从染色聚丙烯酰胺凝胶中进行蛋白质电洗脱的实用且低成本的系统。为此，用玻璃板、1.5毫升容量的微量离心管和透析膜构建了一个水平蛋白质电洗脱槽。对该系统效率的分析表明，从非固定和固定的十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶中可实现高蛋白回收率。