Dyková Iva, Fiala Ivan, Pecková Hana
Institute of Parasitology, Biology Centre, Academy of Sciences of the Czech Republic, Branisovská 31, 370 05 Ceské Budejovice, Czech Republic.
Eur J Protistol. 2008 Nov;44(4):269-77. doi: 10.1016/j.ejop.2008.01.004. Epub 2008 Apr 8.
The molecular phylogeny of Neoparamoeba spp. based on SSU rDNA was updated by including new sequences of strains isolated from an invertebrate and an alga. In total, 59 sequences of strains representating N. pemaquidensis, N. branchiphila, N. aestuarina and N. perurans were analysed. Sequences of SSU rDNA of eukaryotic endosymbionts (Perkinsela amoebae-like organisms) were prepared from 34 samples of genomic DNA of strain-representatives of N. pemaquidensis, N. branchiphila and N. aestuarina. Comparison of phylograms reconstructed from corresponding SSU rDNA sequences of host amoebae and their symbionts revealed a high level of congruence, which argues very strongly for coevolution of these two eukaryotic organisms.
通过纳入从一种无脊椎动物和一种藻类中分离出的菌株的新序列,更新了基于小亚基核糖体DNA(SSU rDNA)的新帕拉变形虫属(Neoparamoeba spp.)的分子系统发育。总共分析了代表佩马奎德新帕拉变形虫(N. pemaquidensis)、鳃栖新帕拉变形虫(N. branchiphila)、河口新帕拉变形虫(N. aestuarina)和秘鲁新帕拉变形虫(N. perurans)的59个菌株序列。从佩马奎德新帕拉变形虫、鳃栖新帕拉变形虫和河口新帕拉变形虫的菌株代表的34个基因组DNA样本中制备了真核内共生体(珀金斯氏菌属变形虫样生物)的SSU rDNA序列。对从宿主变形虫及其共生体相应的SSU rDNA序列重建的系统发育树进行比较,发现了高度的一致性,这有力地证明了这两种真核生物的共同进化。