Meur Gargi, Budatha Madhusudhan, Srinivasan Tantravahi, Rajesh Kumar Koppolu Raja, Dutta Gupta Aparna, Kirti Pulugurtha Bharadwaja
School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
Physiol Plant. 2008 Aug;133(4):765-75. doi: 10.1111/j.1399-3054.2008.01098.x. Epub 2008 Apr 7.
In Arabidopsis, NPR1 (AtNPR1) regulates salicylic acid (SA)-mediated activation of PR genes at the onset of systemic acquired resistance. AtNPR1 also modulates SA-induced suppression of jasmonic acid-responsive gene expression, and npr1 mutants manifest enhanced herbivore resistance. We have raised stable transgenic tobacco lines, expressing AtNPR1 constitutively, which showed elevated expression of PR1 and PR2 genes upon SA treatment. Herbivore bioassays with a generalist polyphagous pest, Spodoptera litura, revealed that the transgenic lines exhibited enhanced resistance compared to the wild-type plants, particularly with respect to younger larval populations. Insect-mediated injury induced several protease inhibitors (PIs), more significantly a 40-kDa serine PI in all the tobacco lines, but the induction was higher in the transgenic plants. We show in this communication that heterologous expression of AtNPR1 provides enhanced resistance to early larval populations of the herbivore, Spodoptera in transgenic tobacco plants.
在拟南芥中，NPR1（AtNPR1）在系统获得性抗性开始时调节水杨酸（SA）介导的PR基因激活。AtNPR1还调节SA诱导的茉莉酸应答基因表达的抑制，并且npr1突变体表现出增强的食草动物抗性。我们培育了稳定表达AtNPR1的转基因烟草品系，这些品系在SA处理后PR1和PR2基因表达升高。用多食性害虫斜纹夜蛾进行的食草动物生物测定表明，与野生型植物相比，转基因品系表现出增强的抗性，特别是对于较年轻的幼虫群体。昆虫介导的损伤诱导了几种蛋白酶抑制剂（PIs），在所有烟草品系中更显著的是一种40 kDa的丝氨酸PI，但转基因植物中的诱导更高。我们在本通讯中表明，AtNPR1的异源表达使转基因烟草植物对食草动物斜纹夜蛾的早期幼虫群体具有增强的抗性。
