[胚胎干细胞分化过程中生物钟基因的表达谱]
[Expression profile of clock genes during the process of embryonic stem cells differentiation].
作者信息
Xie Shu, Cai Yan-ning, Guan Yun-qian, Wu Wen-qi, Xu Yan-ling, Zhang Yu, Chen Biao
机构信息
Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2007 Dec 4;87(45):3203-7.
OBJECTIVE
To analysis the expression profile of7 clock genes during mouse embryonic stem cell (mES) differentiation.
METHODS
Mouse ES cells of the line 129 were cultured and induced to differentiate into neurons by 5 stages method. The expression of 7 clock genes: BMAL1, CLOCK, CRY1, CRY2, PER1, PER2, and PER3 in the five stages were determined by RT-PCR.
RESULTS
In the amplification condition, no non-specific band was found, and only bands of expected sizes were detected. Six clock genes, BMAL1, CLOCK, CRY1, CRY2, PER1 and PER3 were expressed in 129 cells at all five stages in the differentiation process, however, PER2, could only be determined in the last stage.
CONCLUSION
The transcription profile of the clock genes is different during the differentiation of the ES cells towards neurons. The transcription of the gene PER2 is limited to the final stage, when postmitotic neurons and astrocytes emerged. A clock genes loop existing in the mature cells has not been established during the process of ES cell differentiation.
目的
分析7种生物钟基因在小鼠胚胎干细胞（mES）分化过程中的表达谱。
方法
培养129系小鼠胚胎干细胞，采用五阶段法诱导其分化为神经元。通过RT-PCR检测五个阶段中7种生物钟基因：BMAL1、CLOCK、CRY1、CRY2、PER1、PER2和PER3的表达。
结果
在扩增条件下，未发现非特异性条带，仅检测到预期大小的条带。在分化过程的所有五个阶段中，129细胞均表达六种生物钟基因BMAL1、CLOCK、CRY1、CRY2、PER1和PER3，然而，PER2仅在最后阶段可检测到。
结论
在胚胎干细胞向神经元分化过程中，生物钟基因的转录谱不同。基因PER2的转录仅限于有丝分裂后神经元和星形胶质细胞出现的最后阶段。在胚胎干细胞分化过程中尚未建立成熟细胞中存在的生物钟基因环路。
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