顺铂与淋巴因子激活的杀伤细胞联合作用于非小细胞肺癌A549细胞的研究

Combined effect of cisplatin and lymphokine-activated killer cells on A549 cells of non-small cell lung cancer.

作者信息

Zhukova O S, Gerasimova G K, Shubina I Zh, Kiselevskii M V

机构信息

N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow.

出版信息

Bull Exp Biol Med. 2007 Aug;144(2):231-4. doi: 10.1007/s10517-007-0297-2.

DOI:10.1007/s10517-007-0297-2

PMID:18399288

Abstract

We studied the ability of lymphokine-activated killer cells to lyse A549 human non-small cell lung cancer cells after preincubation with cisplatin. Lymphokine-activated killer cells obtained after incubation of human blood lymphocytes with interleukin-2 were characterized by high expression of natural killer cell antigens and activation molecules. Lymphokine-activated killer cells produced potent cytotoxic effect on intact A549 cells and lysed tumor cells survived after treatment with cisplatin in concentrations of IC50 and IC30. Cisplatin in noncytotoxic concentrations did not increase lytic activity of lymphokine-activated killer cells.

摘要

我们研究了淋巴因子激活的杀伤细胞在与顺铂预孵育后裂解A549人非小细胞肺癌细胞的能力。用人血淋巴细胞与白细胞介素-2孵育后获得的淋巴因子激活的杀伤细胞，其特征在于高表达自然杀伤细胞抗原和激活分子。淋巴因子激活的杀伤细胞对完整的A549细胞产生了强大的细胞毒性作用，并裂解了用IC50和IC30浓度的顺铂处理后存活的肿瘤细胞。非细胞毒性浓度的顺铂并未增加淋巴因子激活的杀伤细胞的裂解活性。