Youssef Yahia, Mosleh Ismaili
Suez Canal University, Faculty of Agricultural, Plant Protection Department, Ismailia, Egypt.
Commun Agric Appl Biol Sci. 2007;72(2):117-27.
This study was conducted to investigate the residues of isoproturon and its metabolites, 1-(4-isopropylphenyl)-3-methylurea, 1-(4-isopropylphenyl) urea, and 4-isopropylanilin in soil and mature earthworms under laboratory conditions. Mature earthworms (Aporrectodea caliginosa) were exposed for various durations (7, 15, 30, and 60 days) to soils contaminated with isoproturon concentrations (2, 4, 6, 8, and 10 mg.kg(-1) soil). The decrease in isoproturon concentration in the soil depended on initial concentration it was slower at higher concentrations. The isoproturon and its metabolites accumulated in earthworms it increased during the first 15 days and decreased thereafter. Acute toxicity of isoproturon was determined together with total soluble protein content and glycogen of worms. These parameters were related to isoproturon concentration in soil and earthworms. No lethal effect of isoproturon was observed even at the concentration 1000 mg.kg(-1) soil after 60 days of exposure. A reduction of total soluble protein was observed in all treated worms (maximum 59.54%). This study is suggesting the use of the total soluble protein content and glycogen of earthworms as biomarker of exposure to isoproturon.
本研究旨在调查在实验室条件下，土壤和成熟蚯蚓中异丙隆及其代谢产物1-(4-异丙基苯基)-3-甲基脲、1-(4-异丙基苯基)脲和4-异丙基苯胺的残留情况。将成熟蚯蚓（威廉腔环蚓）暴露于含有不同浓度（2、4、6、8和10 mg.kg(-1)土壤）异丙隆的土壤中，暴露时间分别为7、15、30和60天。土壤中异丙隆浓度的降低取决于初始浓度，在较高浓度下降低速度较慢。异丙隆及其代谢产物在蚯蚓体内积累，在最初15天内增加，之后减少。测定了异丙隆的急性毒性以及蚯蚓的总可溶性蛋白含量和糖原含量。这些参数与土壤和蚯蚓中异丙隆的浓度有关。即使在暴露60天后，土壤浓度达到1000 mg.kg(-1)时，也未观察到异丙隆的致死效应。在所有处理过的蚯蚓中均观察到总可溶性蛋白减少（最多减少59.54%）。本研究表明，可将蚯蚓的总可溶性蛋白含量和糖原含量用作暴露于异丙隆的生物标志物。
