Paya P, Mulero J, Oliva J, Camara M A, Zafrilla P, Barba A
Departamento Química Agrícola, Geología y Edafología, Facultad de Química, Universidad de Murcia, Campus de Espinardo, s/n, 30100, Espinardo, Murcia, Spain.
Commun Agric Appl Biol Sci. 2007;72(2):151-9.
The aim of this study was to offer data about the bioavailability of flufenoxuron, lufenuron, pyriproxyfen and fenoxycarb in common commodities like mandarin, apricot and peach. The in vitro bioavailability of the compounds was studied not only in fresh fruit but also in standards and canned food in order to establish possible differences according to the matrix. The gastric digestion was simulated with porcine pepsin at pH 2, for 2 h in a shaking water bath at 37 degrees C. The intestinal digestion was simulated with porcine pancreatin at pH 7, for 2 h in a shaking water bath at 37 degrees C. The intestinal absorption was simulated with cellulose dialysis tubing filled with a solution of sodium carbonate. No in vitro bioavailability was observed in mandarin, peach and apricot samples spiked at the concentrations generally found in the market for the raw and processed commodities. In standards, the dialysis started at the level of 0.25 mg/kg. This is an approximation to the pesticide digestion and absorption in humans.
本研究的目的是提供氟虫脲、虱螨脲、吡丙醚和苯氧威在柑橘、杏和桃等常见商品中的生物利用度数据。为了确定根据基质可能存在的差异，不仅在新鲜水果中，而且在标准品和罐头食品中研究了这些化合物的体外生物利用度。在pH 2的条件下，用猪胃蛋白酶在37℃的振荡水浴中模拟胃消化2小时。在pH 7的条件下，用猪胰酶在37℃的振荡水浴中模拟肠道消化2小时。用填充有碳酸钠溶液的纤维素透析管模拟肠道吸收。在添加了生鲜和加工商品市场上常见浓度的柑橘、桃和杏样品中，未观察到体外生物利用度。在标准品中，透析从0.25 mg/kg的水平开始。这是对农药在人体中消化和吸收的一种近似模拟。
