Ramos-Zúñiga R, Gómez P U, Navarro Ruiz A, Luquín de A S, García-Estrada J
Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara Jalisco, México.
Minim Invasive Neurosurg. 2008 Apr;51(2):87-90. doi: 10.1055/s-2007-1022531.
The Mongolian gerbil is one of the main animal species used for the study of global ischemia, due to its specific Circle of Willis. Because of their anatomic variations, a large number of animals is needed. On account of the specific vulnerability of the hippocampus, striatum and neocortex, it is possible to evaluate the severity of the ischemic damage through an analysis of locomotor activity. The tests support the sensitvity of the experimental sample and compensate the interanimal variability.
The locomotor pattern of 30 male Mongolian gerbils was recorded before they were subjected to experimental bilateral carotid clippage for 15 minutes followed by reperfusion. A transparent 75x50x90 cm acrylic box was filmed in order to determine the total distance covered by the animals in five minutes, for three consecutive days. The locomotor activity of the animals was also examined in an open field at 24 hours and seven days after ischemia. Serum neurospecific enolase (NSE) was measured in the ischemic group and compared with that of an intact control group.
The recording for normal animals was uniformly similar (average 200 squares in periphery), in the first trial of 3 consecutive days (188+/-6.7 S.D.). After ischemia, the numbers increased to 388 (+/- 40 S.D.), indicating that they were sensitive to the ischemic episode. Seven days later they returned to basal values. Serum NSE was high in the ischemic group versus the intact control group (S=<0.001).
Locomotor activity in an open field is a useful reference as a predictive test to determine the sensitivity of experimental animals to ischemia. It is also associated to the degree of cerebral damage in global ischemia-reperfusion, and this behavior is representative of the expression of selective ischemic injury. The determination of NSE is useful as an associated parameter of ischemic injury.
蒙古沙鼠因其特定的 Willis 环，是用于全球缺血研究的主要动物物种之一。由于其解剖变异，需要大量动物。鉴于海马体、纹状体和新皮质的特定易损性，通过分析运动活动来评估缺血损伤的严重程度是可行的。这些测试支持实验样本的敏感性并补偿动物间的变异性。
记录 30 只雄性蒙古沙鼠在进行双侧颈动脉夹闭 15 分钟然后再灌注的实验前的运动模式。拍摄一个 75x50x90 厘米的透明丙烯酸箱，以确定动物在连续三天内五分钟内覆盖的总距离。还在缺血后 24 小时和七天在开放场地检查动物的运动活动。测量缺血组的血清神经特异性烯醇化酶（NSE）并与完整对照组进行比较。
连续三天的第一次试验中，正常动物的记录一致相似（外周平均 200 格）（188±6.7 标准差）。缺血后，数量增加到 388（±40 标准差），表明它们对缺血事件敏感。七天后它们恢复到基础值。与完整对照组相比，缺血组的血清 NSE 较高（P<0.001）。
开放场地中的运动活动作为一种预测性测试，对于确定实验动物对缺血的敏感性是一种有用的参考。它还与全球缺血再灌注中的脑损伤程度相关，并且这种行为代表了选择性缺血损伤的表达。NSE 的测定作为缺血损伤的相关参数是有用的。
