Guvenc Tolga, Yarim Murat, Gulbahar Mustafa Yavuz, Kabak Yonca B
Department of Pathology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Kurupelit, 55139 Samsun, Turkey.
Acta Vet Hung. 2008 Mar;56(1):117-23. doi: 10.1556/AVet.56.2008.1.12.
The cerebella of 12 dogs infected with canine distemper virus (CDV) and those of three normal dogs were examined. The avidin-biotin-peroxidase complex technique was used to detect alphaB-crystallin (alphaB-c) immunoreactivity and immunolocalisation of the CDV antigen. CDV antigens, immunopositive astrocytes, oligodendrocytes and granular neurons were seen in both the white and grey matter of the infected dogs. In the controls, alphaB-c immunopositive glial cells were seen in the white matter and around the Purkinje cells. In dogs with distemper, alphaB-c immunoreactivity was not observed in some of the glial cells around the Purkinje cells. A significant negative correlation of P < 0.01 level was found between areas of severe demyelination and the number of alphaB-c immunopositive cells in dogs infected with CDV. Such correlation was not observed between mild and moderate demyelinating areas and alphaB-c immunostaining. The alphaB-crystallin/ total number of cells ratio was found to be significant in severely affected demyelinating areas (P < 0.05). These data indicate that there was a relationship between the degrees of CDV associated with demyelination and the level of alphaB-c expression in the glial cells.
对12只感染犬瘟热病毒（CDV）的犬以及3只正常犬的小脑进行了检查。采用抗生物素蛋白-生物素-过氧化物酶复合物技术检测αB-晶状体蛋白（αB-c）的免疫反应性以及CDV抗原的免疫定位。在感染犬的白质和灰质中均可见到CDV抗原、免疫阳性的星形胶质细胞、少突胶质细胞和颗粒神经元。在对照组中，白质以及浦肯野细胞周围可见αB-c免疫阳性的神经胶质细胞。在患犬瘟热的犬中，浦肯野细胞周围的一些神经胶质细胞未观察到αB-c免疫反应性。在感染CDV的犬中，严重脱髓鞘区域与αB-c免疫阳性细胞数量之间存在显著的负相关（P<0.01）。在轻度和中度脱髓鞘区域与αB-c免疫染色之间未观察到这种相关性。在严重受影响的脱髓鞘区域，αB-晶状体蛋白/细胞总数的比率具有显著性（P<0.05）。这些数据表明，与脱髓鞘相关的CDV程度与神经胶质细胞中αB-c的表达水平之间存在关联。
