Grases Félix, Sanchis Pilar, Costa-Bauzá Antonia, Bonnin Oriol, Isern Bernat, Perelló Joan, Prieto Rafael M
Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS), University of Balearic Islands, Palma of Mallorca, Spain.
Cardiovasc Pathol. 2008 May-Jun;17(3):139-45. doi: 10.1016/j.carpath.2007.08.005. Epub 2007 Oct 24.
The present study examined the inhibitory effects of pyrophosphate, etidronate, and phytate on bovine pericardium calcification in vitro.
Bovine pericardium was glutaraldehyde fixed and then placed in a flow chamber in the presence of a synthetic physiological fluid alone (control) or the fluid plus various concentrations of pyrophosphate, etidronate, or phytate. Following a 96-h incubation, fragments were removed and assayed for calcification by measuring calcium and phosphorus levels.
The data indicated that both pyrophosphate and etidronate at 1 mg/l (5.75 and 4.95 microM, respectively) inhibited bovine pericardium calcification, whereas neither agent had an effect at 0.5 mg/l (2.87 and 2.47 microM, respectively). Phytate was the most potent inhibitor of calcification, and the effects of this agent were apparent at levels as low as 0.25 mg/l (0.39 microM).
While pyrophosphate, etidronate, and phytate were all able to inhibit bovine pericardium calcification in vitro, phytate was found to be the most effective.
本研究检测了焦磷酸盐、依替膦酸盐和植酸对牛心包钙化的体外抑制作用。
牛心包经戊二醛固定，然后置于流动腔中，分别在仅含合成生理液（对照）或含不同浓度焦磷酸盐、依替膦酸盐或植酸的生理液中培养。孵育96小时后，取出碎片，通过测量钙和磷水平检测钙化情况。
数据表明，1mg/l（分别为5.75和4.95微摩尔）的焦磷酸盐和依替膦酸盐均可抑制牛心包钙化，而0.5mg/l（分别为2.87和2.47微摩尔）时两种药物均无作用。植酸是最有效的钙化抑制剂，其在低至0.25mg/l（0.39微摩尔）的水平时作用就很明显。
虽然焦磷酸盐、依替膦酸盐和植酸均可在体外抑制牛心包钙化，但发现植酸最为有效。
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