Stodůlka J
Department of Experimental Neurology, Medical Faculty, Palacký University, Olomouc, Czechoslovakia.
Acta Univ Palacki Olomuc Fac Med. 1991;131:39-81.
The paper is aimed at the evaluation the effect of relatively low doses of ethanol and physostigmine salicylate on behavior of the male Wistar rat in an open field test. Both drugs were administered as a solution intragastrically 1 hour before the test started in dose ranges of 0.6-1.8 g/kg and 0.06-0.18 mg/kg, respectively. Lower doses of ethanol showed dose dependent biphasic effect on the ambulation and grooming time in animals. The medium dose 1.2 g/kg was rather ineffective in affecting rat behavior in the hole board test. Individual variables showed different sensitivity to individual ethanol doses. But from the medium dose, individual manifestations of ataxia were observed. Using the dose, only total ambulation, rearing time and rear latency were not affected when the rats were experienced with repeated exposure to open field arena or ethanol dose beforehand. Physostigmine alone increased defecation level significantly by two doses but lower doses changed some of the other variables, too. Combined administration of both drugs in medium doses did not affect the rat behavior considerably. The intra-trial habituation of three parameters of OF behavior seems to be influenced differently. Ethanol showed a tendency to facilitate the habituation of rearing, but rather to interfere with that of grooming. The later effect was also shown for physostigmine 0.12 mg/kg. The experimental part of the paper is supplied by the discussion of aspects important for modulation of rodent behavior in an open field test.
本文旨在评估相对低剂量的乙醇和水杨酸毒扁豆碱对雄性Wistar大鼠在旷场试验中的行为影响。两种药物均在试验开始前1小时以溶液形式灌胃给药，剂量范围分别为0.6 - 1.8 g/kg和0.06 - 0.18 mg/kg。较低剂量的乙醇对动物的行走和梳理时间呈现剂量依赖性双相效应。中等剂量1.2 g/kg在洞板试验中对大鼠行为的影响相当小。个体变量对不同乙醇剂量表现出不同的敏感性。但从中等剂量开始，观察到共济失调的个体表现。使用该剂量时，当大鼠预先经历过反复暴露于旷场区域或乙醇剂量时，只有总行走、竖毛时间和竖毛潜伏期不受影响。单独使用水杨酸毒扁豆碱时，两个剂量显著增加了排便水平，但较低剂量也改变了一些其他变量。两种药物中等剂量联合给药对大鼠行为的影响不大。旷场行为三个参数的试验内习惯化似乎受到不同影响。乙醇表现出促进竖毛习惯化的趋势，但却倾向于干扰梳理习惯化。0.12 mg/kg的水杨酸毒扁豆碱也表现出后一种效应。本文的实验部分还讨论了在旷场试验中对啮齿动物行为调节重要的方面。
