Tsutsumi Takaki, Ide Tomomi, Yamato Mayumi, Andou Makoto, Shiba Takeshi, Utsumi Hideo, Sunagawa Kenji
Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Free Radic Res. 2008 Apr;42(4):305-11. doi: 10.1080/10715760801986542.
Although the advent of in vivo electron spin resonance (ESR) spectroscopy has allowed analysis of the redox status of living animals, whether the haemodynamic condition affects the signal decay rate remains unknown. Three kinds of haemodynamic conditions were generated by changing the anaesthetic dosage in mice. Haemodynamics was analysed (n=6 each) and in vivo ESR was performed to measure the signal decay rates of three nitroxyl spin probes (carbamoyl-, carboxy- and methoxycarbonyl-PROXYL) at the chest and head regions (n=6 for each condition and probe). Haemodynamic analysis revealed negative inotropic and chronotropic effects on the cardiovascular system depending on the depth of anaesthesia. Although signal decay rates differed among three probes, they were not affected by heart rate alteration. In this study we report the haemodynamics-independent signal decay rate of nitoxyl probes.
尽管体内电子自旋共振（ESR）光谱技术的出现使得对活体动物的氧化还原状态进行分析成为可能，但血流动力学状况是否会影响信号衰减率仍不清楚。通过改变小鼠的麻醉剂量产生了三种血流动力学状况。分析了血流动力学（每组n = 6），并进行了体内ESR测量，以测定三种硝酰基自旋探针（氨基甲酰基、羧基和甲氧基羰基-PROXYL）在胸部和头部区域的信号衰减率（每种状况和探针每组n = 6）。血流动力学分析显示，根据麻醉深度不同，对心血管系统有负性肌力和负性变时作用。尽管三种探针的信号衰减率有所不同，但它们不受心率变化的影响。在本研究中，我们报告了硝酰基探针与血流动力学无关的信号衰减率。
