Leal Luzia K, Fonseca Francisco Noé, Pereira Fábio Azevedo, Canuto Kirley M, Felipe Cícero F, Fontenele Juvênia Bezerra, Pitombeira Márcia V, Silveira Edilberto R, Viana Glauce S
Departmento de Farmácia, Universidade Federal do Ceará, Rua Capitão Francisco Pedro 1210, Fortaleza, Brazil.
Planta Med. 2008 Apr;74(5):497-502. doi: 10.1055/s-2008-1074501. Epub 2008 Apr 10.
The aim of this study was to investigate the possible beneficial effects of amburoside A, AMB [4-(O-beta- D-glycopyranosyl)benzyl protocatechoate], against carbon tetrachloride (CCl (4)) toxicity in rats. AMB is a phenol glucoside from the Brazilian medicinal plant Amburana cearensis, popularly used for the treatment of respiratory tract affections. Acute AMB (25 and 50 mg/kg, I. P. or P. O.) treatments of CCl (4)-intoxicated rats significantly inhibited the increase in serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, as compared to the group treated with CCl (4) only. Histological studies showed less centrolobular necrosis and inflammatory cell infiltrates in the liver of animals treated with AMB plus CCl (4), when compared to the group treated with CCl (4) alone. In hepatic tissues, AMB at both doses inhibited CCl (4)-induced thiobarbituric acid-reactive substances (TBARS) formation, indicating a blockade of CCl (4)-induced lipid peroxidation. AMB also reversed the decrement in glutathione contents of hepatic tissues in CCl (4)-intoxicated rats. Furthermore, it restored catalase activity to normal values, which was significantly increased after CCl (4) treatment. Our results indicate that CCl (4)-induced oxidative damage in hepatic tissues is reversed by AMB treatment. The protective effect of AMB is probably due to the phenolic nature of this glucoside.
本研究旨在探讨ambuoside A（AMB，4-(O-β-D-吡喃葡萄糖基)原儿茶酸苄酯）对大鼠四氯化碳（CCl₄）毒性可能的有益作用。AMB是一种来自巴西药用植物巴西苏木的酚类糖苷，常用于治疗呼吸道疾病。与仅用CCl₄处理的组相比，用急性AMB（25和50 mg/kg，腹腔注射或口服）处理CCl₄中毒的大鼠可显著抑制血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平的升高。组织学研究表明，与单独用CCl₄处理的组相比，用AMB加CCl₄处理的动物肝脏中央小叶坏死和炎性细胞浸润较少。在肝组织中，两种剂量的AMB均抑制CCl₄诱导的硫代巴比妥酸反应性物质（TBARS）形成，表明阻断了CCl₄诱导的脂质过氧化。AMB还逆转了CCl₄中毒大鼠肝组织中谷胱甘肽含量的减少。此外，它将过氧化氢酶活性恢复到正常值，CCl₄处理后该活性显著增加。我们的结果表明，AMB处理可逆转CCl₄诱导的肝组织氧化损伤。AMB的保护作用可能归因于该糖苷的酚类性质。
