Oresković Darko, Maraković Jurica, Vukić Miroslav, Rados Milan, Klarica Marijan
Laboratory of Neurochemistry and Molecular Neurobiology, Department of Molecular Biology, Ruder Boskovic Institute, Zagreb, Croatia.
Coll Antropol. 2008 Jan;32 Suppl 1:133-7.
The aim of the study was to evaluate whether or not cerebrospinal fluid formation rate (Vf) calculated according to the equation of Heisey et al., truly show the produced cerebrospinal fluid. For this reason Vf was simulated (40.6 microL/min) by an infusion pump in a plastic cylinder and the evaluation was done by comparing the results obtained between the calculated Vf and the simulated one. In both cases the result should be the same (40.6 micro/min). Other types of experiments were carried out by ventriculocisternal perfusion (92.4 microL/min) on anaesthetized and sacrificed cats. If the equation is correct, the calculated Vf for sacrificed animals should be zero, because there is no Vf in dead animals. The fact that the calculated Vf (46.5 microL/min) in the plastic cylinder was different (p < 0.0001) from the simulated one (40.6 microL/min) and that Vf was calculated even for dead animals (3-5 microL/min) clearly shows the that perfusion method may not be an accurate method for determination of Vf.
本研究的目的是评估根据海西等人的公式计算出的脑脊液生成率（Vf）是否真的能反映所产生的脑脊液。因此，在一个塑料圆柱体内用输液泵模拟了Vf（40.6微升/分钟），并通过比较计算得出的Vf与模拟值之间的结果进行评估。在这两种情况下，结果应该是相同的（40.6微升/分钟）。还对麻醉并处死后的猫进行了脑室脑池灌注（92.4微升/分钟）等其他类型的实验。如果该公式正确，那么处死后动物的计算Vf应该为零，因为死亡动物不存在脑脊液生成。塑料圆柱体内计算得出的Vf（46.5微升/分钟）与模拟值（40.6微升/分钟）不同（p<0.0001），而且甚至对死亡动物也计算出了Vf（3 - 5微升/分钟），这清楚地表明灌注法可能不是测定Vf的准确方法。
