Fu De-yu, Wang Shi-hong, Zhou Duan, Ma Yu-ying, Jin Lu, Zu Liang-hua
Department of Cardiology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Zhong Xi Yi Jie He Xue Bao. 2008 Apr;6(4):387-91. doi: 10.3736/jcim20080412.
To explore the possible mechanism of Huoxue Qianyang Formula (HXQYF), a compound traditional Chinese herbal medicine, in reversing the left ventricular hypertrophy (LVH) of spontaneous hypertensive rats (SHRs) by analyzing the expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle.
The experimental study was carried out in SHRs, the sex- and age-matched Wistar-Kyoto (WKY) rats were served as normal control (n=5, normal saline 10 ml/kg daily). Twenty-five SHRs were randomly divided into five groups: untreated group (n=5, normal saline 10 ml/kg daily), high-dose HXQYF-treated group (n=5, 0.84 g/ml HXQYF, 10 ml/kg daily), medium-dose HXQYF-treated group (n=5, 0.42 g/ml HXQYF, 10 ml/kg daily), low-dose HXQYF-treated group (n=5, 0.21 g/ml HXQYF, 10 ml/kg daily) and cilazapril-treated group (n=5, 1 mg/ml cilazapril, 10 ml/kg daily). The drugs were intragastrically administered once daily for 14 weeks. The expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle were detected separately by in situ hybridization histochemical method and immunohistochemical method.
Compared with the normal control group, the expressions of mRNAs and proteins of proto-oncogenes c-fos and c-myc in left ventricular muscle were significantly increased in untreated group (P<0.01). After treatment, the expressions of c-fos and c-myc mRNAs in left ventricular muscle in HXQYF-treated groups were significantly down-regulated as compared with those of the untreated group (P<0.05). The expressions of c-myc protein were also significantly decreased in high- and medium-dose HXQYF-treated groups as compared with the untreated group (P<0.05), but it had no significant effects in protein expression of c-fos in the three HXQYF-treated groups.
HXQYF can inhibit the expression of c-myc in ventricular hypertrophy tissue, which may be the mechanism in treating LVH of hypertension.
通过分析原癌基因c-fos和c-myc在左心室肌中的mRNA和蛋白表达，探讨中药复方活血潜阳方（HXQYF）逆转自发性高血压大鼠（SHR）左心室肥厚（LVH）的可能机制。
以SHR为实验对象，选取性别和年龄匹配的Wistar-Kyoto（WKY）大鼠作为正常对照（n = 5，每日给予生理盐水10 ml/kg）。将25只SHR随机分为5组：未治疗组（n = 5，每日给予生理盐水10 ml/kg）、高剂量HXQYF治疗组（n = 5，0.84 g/ml HXQYF，每日10 ml/kg）、中剂量HXQYF治疗组（n = 5，0.42 g/ml HXQYF，每日10 ml/kg）、低剂量HXQYF治疗组（n = 5，0.21 g/ml HXQYF，每日10 ml/kg）和西拉普利治疗组（n = 5，1 mg/ml西拉普利，每日10 ml/kg）。药物每日灌胃1次，连续14周。分别采用原位杂交组织化学法和免疫组织化学法检测左心室肌中原癌基因c-fos和c-myc的mRNA和蛋白表达。
与正常对照组相比，未治疗组左心室肌中原癌基因c-fos和c-myc的mRNA和蛋白表达显著升高（P < 0.01）。治疗后，HXQYF治疗组左心室肌中c-fos和c-myc mRNA的表达与未治疗组相比显著下调（P < 0.05）。高、中剂量HXQYF治疗组c-myc蛋白表达与未治疗组相比也显著降低（P < 0.05），但HXQYF治疗的3组中c-fos蛋白表达无显著变化。
HXQYF可抑制心室肥厚组织中c-myc的表达，这可能是其治疗高血压左心室肥厚的机制。
