Boros Csaba, Lukácsi Erika, Horváth-Oszwald Erzsébet, Réthelyi Miklós
Szentágothai János Laboratory, Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Tuzoltó utca 58, Hungary.
Brain Res. 2008 May 13;1209:105-14. doi: 10.1016/j.brainres.2008.02.029. Epub 2008 Feb 21.
Contrary to the widespread assumption, the filum terminale in the rat possesses a precise glial and neuronal organization. The processes of glial fibrillary acidic protein-stained astrocytes form a rich, three dimensional array. The crescent shaped white matter could be outlined with antibody detecting oligodendrocytes. The neurons in the filum terminale, labeled with neuron-specific nuclear protein, are distributed in a small midline group (dorsal nucleus) dorsal to and in two symmetrical clusters at both sides of the central canal (lateral nuclei). Nitric oxide synthase-, calretinin-, choline acetyltransferase-, substance P- and neurokinin receptor-1-immunoreactive neurons were detected in the lateral nuclei. Axons were classified based on their course and termination. Small number of calcitonin gene-related peptide-immunoreactive fibers was found exclusively in the dorsal nucleus. Nitric oxide synthase-, substance P-, and neurokinin receptor-1-stained axon arborizations were detected mainly in the lateral nucleus. A dense array of extremely fine vesicular glutamate transporter 2- and fine, synaptophysin-immunoreactive varicosities covered densely the lateral nuclei. Fine glycine-transporter 2-immunoreactive axon arborization like structures were seen also in the lateral nucleus. Vesicular glutamate transporter 1- and choline acetyltransferase-immunoreactive axons arborized in the entire gray matter. Serotonin- and enkephalin-immunoreactive fibers congregated in the dorsolateral portion of the white matter, called "shoulder region", while calretinin- and thick, varicose neurokinin receptor-1-stained axons were also seen in the same area of the white matter. Synaptophysin-immunoreactive fine varicosities colocalized only with vesicular glutamate transporter 2 immunoreaction. Substance P and glycine-transporter 2-immunoreactive puncta were found in close contact with neurokinin receptor-1-immunostained perikarya and dendrites.
与普遍的假设相反，大鼠终丝具有精确的神经胶质和神经元组织。胶质纤维酸性蛋白染色的星形胶质细胞的突起形成丰富的三维阵列。用检测少突胶质细胞的抗体可以勾勒出新月形的白质。用神经元特异性核蛋白标记的终丝中的神经元分布在中央管背侧的一个小中线组（背核）以及中央管两侧的两个对称簇（侧核）中。在侧核中检测到一氧化氮合酶、钙视网膜蛋白、胆碱乙酰转移酶、P物质和神经激肽受体-1免疫反应性神经元。轴突根据其行程和终末进行分类。少量降钙素基因相关肽免疫反应性纤维仅在背核中发现。一氧化氮合酶、P物质和神经激肽受体-1染色的轴突分支主要在侧核中检测到。密集排列的极细囊泡谷氨酸转运体2和细的、突触素免疫反应性膨体密集覆盖侧核。在侧核中也可见到细的甘氨酸转运体2免疫反应性轴突分支样结构。囊泡谷氨酸转运体1和胆碱乙酰转移酶免疫反应性轴突在整个灰质中分支。5-羟色胺和脑啡肽免疫反应性纤维聚集在白质的背外侧部分，称为“肩部区域”，而在白质的同一区域也可见到钙视网膜蛋白和粗的、曲张的神经激肽受体-1染色的轴突。突触素免疫反应性细膨体仅与囊泡谷氨酸转运体2免疫反应共定位。P物质和甘氨酸转运体2免疫反应性小点与神经激肽受体-1免疫染色的胞体和树突紧密接触。
