Reichwald Kirsten, Hatzack Frank
Novozymes A/S, Laurentsvej 55, DK-2880 Bagsvaerd, Denmark.
J Agric Food Chem. 2008 May 14;56(9):2888-91. doi: 10.1021/jf0730690. Epub 2008 Apr 12.
A modified version of the Haug and Lantzsch method for rapid photometrical phytate determination was applied for the analysis of phytate in soybean, wheat, and maize meals. In comparison to the original protocol, the amount of the toxic reagent thioglycolic acid is reduced substantially to minimize potential health risks for laboratory personnel. Different extraction conditions for soybean meal were tested, and boiling for at least 30 min was found to be necessary to remove an interfering compound in soybean meal extracts. Phytate contents determined according to the modified Haug and Lantzsch method did not differ from those obtained by measuring total precipitated phosphorus or by sensitive and specific high-performance ion chromatography. Applicability and accuracy of the modified method for phytate analysis in major feed substrates, including soy-based textured vegetable protein, were demonstrated.
采用一种改良的豪格和兰茨施快速光度法测定植酸盐的方法，对大豆、小麦和玉米粉中的植酸盐进行分析。与原始方案相比，有毒试剂巯基乙酸的用量大幅减少，以将实验室人员的潜在健康风险降至最低。对大豆粉的不同提取条件进行了测试，发现至少煮沸30分钟对于去除大豆粉提取物中的干扰化合物是必要的。根据改良的豪格和兰茨施方法测定的植酸盐含量，与通过测量总沉淀磷或灵敏且特异的高效离子色谱法获得的含量没有差异。证明了该改良方法在包括大豆基组织化植物蛋白在内的主要饲料底物中分析植酸盐的适用性和准确性。
