Sutherland I A, Damsteegt A, Miller C M, Leathwick D M
AgResearch, Hopkirk Institute, Palmerston North, New Zealand.
N Z Vet J. 2008 Apr;56(2):67-70. doi: 10.1080/00480169.2008.36810.
To confirm the presence of multiple anthelmintic resistance on a sheep farm in New Zealand.
Three groups of 10 weaned Romney-cross lambs were treated either with an oral dose of ivermectin (0.2 mg/kg), or a benzimidazole/levamisole (BZ/LEV) combination (4.75 albendazole and 7.5 mg/kg levamisole), or were left untreated. Ten days later, animals were necropsied, and adult worms recovered and identified from the abomasa and small intestines. Pre- and post-treatment faecal nematode egg counts (FEC) were recorded, and larval cultures were performed.
In a faecal egg count reduction test (FECRT), adjusted to reflect pre- and post-treatment larval culture results, ivermectin resistance was detected in Teladorsagia (Ostertagia), Trichostrongylus and Haemonchus spp, while BZ/LEV combination- resistant Teladorsagia and Trichostrongylus spp were also present. Adult worm counts confirmed these results, and identified the species involved as Teladorsagia circumcincta, Trichostrongylus colubriformis and H. contortus.
Multiple, multi-generic anthelmintic resistance was confirmed on a sheep property in New Zealand. This included the first confirmed case of ivermectin resistance in T. colubriformis from sheep in New Zealand.
确认新西兰一个养羊场存在多种抗蠕虫药耐药性。
将三组各10只断奶的罗姆尼杂交羔羊分别口服伊维菌素（0.2毫克/千克），或苯并咪唑/左旋咪唑（BZ/LEV）组合（4.75毫克阿苯达唑和7.5毫克/千克左旋咪唑），或不进行治疗。10天后，对动物进行尸体剖检，从皱胃和小肠中回收并鉴定成虫。记录治疗前后的粪便线虫卵计数（FEC），并进行幼虫培养。
在粪便虫卵计数减少试验（FECRT）中，根据治疗前后幼虫培养结果进行调整后，检测到对伊维菌素耐药的环纹奥斯特线虫（捻转血矛线虫）、毛圆线虫属和捻转血矛线虫，同时也存在对BZ/LEV组合耐药的环纹奥斯特线虫和毛圆线虫属。成虫计数证实了这些结果，并确定涉及的物种为环纹奥斯特线虫、蛇形毛圆线虫和捻转血矛线虫。
在新西兰的一个养羊场确认存在多种、多属的抗蠕虫药耐药性。这包括新西兰首次确认的绵羊蛇形毛圆线虫对伊维菌素耐药的病例。
