Hibi Masanobu, Takase Hideto, Yasunaga Koichi, Yamaguchi Tohru, Harada Ushio, Katsuragi Yoshihisa, Tokimitsu Ichiro
Health Care Food Research Laboratories, Kao Corporation, 2-1-3, Bunka, Tokyo, 131-8501, Japan.
Lipids. 2008 Jun;43(6):517-24. doi: 10.1007/s11745-008-3177-2. Epub 2008 Apr 12.
Several studies in animals and humans have reported beneficial effects of diacylglycerol (DAG) on lipid and energy metabolism. We assessed the effect of DAG versus triacylglycerol (TAG) treatment on total energy expenditure (TEE), total fat oxidation (Fox) and respiratory quotient (RQ), and measured the oxidation rate of each oil using a respiratory chamber and the 13C-stable isotope. Eleven healthy subjects participated in a double-blind, randomized crossover study. Subjects consumed an energy maintenance diet consisting of 55% of total calories from carbohydrate, 15% from protein and 30% from fat during both the 3-day pre-chamber and 36-h chamber period. Fifty percent of the fat was test oil, containing either DAG oil or TAG oil. The oxidation rate of ingested test oils was determined by monitoring 13CO2 excretion in the breath from 13C-labeled diolein or 13C-labeled triolein. There were no significant differences in TEE, RQ and total Fox between the DAG and TAG treatment in the overall analysis. In the subgroup analysis, DAG treatment decreased RQ significantly in subjects with a high fat ratio (HFR) compared to TAG treatment. In addition, ingested diolein oxidation in DAG treatment was significantly faster than triolein oxidation in TAG treatment in the HFR group. Enhanced fat utilization with DAG treatment and rapid oxidation of ingested DAG may, at least in part, explain the greater loss of body weight and body fat related to DAG consumption found in the weight-loss studies.
多项针对动物和人类的研究报告了二酰甘油（DAG）对脂质和能量代谢的有益影响。我们评估了DAG与三酰甘油（TAG）处理对总能量消耗（TEE）、总脂肪氧化（Fox）和呼吸商（RQ）的影响，并使用呼吸室和13C稳定同位素测量了每种油的氧化率。11名健康受试者参与了一项双盲、随机交叉研究。在3天的呼吸室前期和36小时的呼吸室期，受试者均食用一种能量维持饮食，其中碳水化合物提供总热量的55%，蛋白质提供15%，脂肪提供30%。脂肪的50%为试验油，包含DAG油或TAG油。通过监测呼出气体中13C标记的二油精或13C标记的三油精产生的13CO2排泄量来确定摄入试验油的氧化率。在总体分析中，DAG和TAG处理之间的TEE、RQ和总Fox没有显著差异。在亚组分析中，与TAG处理相比，DAG处理使高脂肪比例（HFR）受试者的RQ显著降低。此外，在HFR组中，DAG处理时摄入的二油精氧化明显快于TAG处理时的三油精氧化。DAG处理增强脂肪利用以及摄入的DAG快速氧化，可能至少部分解释了在减肥研究中发现的与食用DAG相关的更大体重和体脂损失。
