在人类和公牛精液中成功分离出X、Y染色体及精子。

Successful separation of X and Y and spermatozoa in human and bull semen.

作者信息

Bhattacharya B C, Shome P, Gunther A H, Evans B M

出版信息

Int J Fertil. 1977;22(1):30-5.

Abstract

Using particle free semen extender and applying a low temperature thermal convection laminar flow to bring the sperm cells in vertical orientation and allowing them to act through the center of buoyancy, a bimodal distribution was achieved. Laser scanner for observing sedimentation pattern and F-body technique for identification of male sperm were used. Specimens from five human donors and ten bulls were separated, checked for purity, and frozen in liquid nitrogen. The lighter fractions contained 59.4% male (P less than 0.01), the heavier fractions 63.5% female (P less than 0.01), and the nonprocessed semen 48.0% male cells. Over 10,000 cells were counted to arrive at this statistically significant result. Observed sex ratio closely matched the predicted purity figures. Lighter and heavier fractions were further purified using forced convection galvanic method. Seventy to eighty percent purity in both fractions was confirmed by F-body and small cell galvanic migration tests with human as well as with bull semen.

摘要

使用无颗粒精液稀释剂，并采用低温热对流层流使精子细胞垂直排列，让它们通过浮力中心起作用，从而实现了双峰分布。使用了激光扫描仪来观察沉降模式，并采用F体技术来鉴定雄性精子。分离了来自五名人类供体和十头公牛的样本，检查其纯度，然后在液氮中冷冻。较轻的部分含有59.4%的雄性（P小于0.01），较重的部分含有63.5%的雌性（P小于0.01），而未处理的精液含有48.0%的雄性细胞。为了得出这一具有统计学意义的结果，对超过10000个细胞进行了计数。观察到的性别比例与预测的纯度数据非常匹配。使用强制对流电流法对较轻和较重的部分进一步纯化。通过对人类和公牛精液进行F体和小细胞电流迁移测试，证实了两个部分的纯度均达到70%至80%。