禽蛋清蛋白提取物的抗菌活性比较

Comparative antibacterial activity of avian egg white protein extracts.

Wellman-Labadie O, Picman J, Hincke M T

Department of Biology, University of Ottawa, Ottawa, Ontario.

Br Poult Sci. 2008 Mar;49(2):125-32. doi: 10.1080/00071660801938825.

Abstract

Egg white proteins from the eggs of domestic chicken (Gallus gallus), turkey (Meleagris gallopavo), duck (Anas platyrhynchos) and goose (Anser anser) were analysed in order to compare the antimicrobial activity of these products. 2. Albumen from each species was sampled and analysed by SDS-PAGE and Western blotting. Antimicrobial activity and lysozyme activity were measured. 3. Ovotransferrin and ovalbumin were identified in all species while c-type lysozyme was present in chicken, turkey and duck egg white samples, but not in goose. 4. Galliformes appear to possess albumens with greater antimicrobial activity than those of the Anseriformes. This can be attributed to higher concentrations of ovotransferrin and the broad acting c-type lysozyme.

摘要

为了比较家鸡（原鸡）、火鸡、鸭和鹅所产鸡蛋的蛋清蛋白的抗菌活性，对这些鸡蛋的蛋清蛋白进行了分析。2. 对每个物种的蛋清进行采样，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质免疫印迹法进行分析。测量了抗菌活性和溶菌酶活性。3. 在所有物种中均鉴定出卵转铁蛋白和卵清蛋白，而c型溶菌酶存在于鸡、火鸡和鸭的蛋清样品中，但鹅蛋清中没有。4. 鸡形目动物的蛋清似乎比雁形目动物的蛋清具有更强的抗菌活性。这可归因于卵转铁蛋白的较高浓度和作用广泛的c型溶菌酶。