Gajewska Joanna, Chełchowska Magdalena, Ceran Alicja, Ambroszkiewicz Jadwiga, Laskowska-Klita Teresa, Szymański Maciej, Barciszewski Jan
Zaktad Badań Przesiewowych, Pracownia Biochemii Instytutu Matki i Dziecka w Warszawie.
Przegl Lek. 2007;64(10):671-3.
An improvement in prenatal screening for chromosomal defects in the first trimester of pregnancy has been achieved by combining sonography and biochemical markers. Among these markers exist the metalloproteinase pregnancy-associated plasma protein-A (PAPP-A) which promotes cell growth by proteolytic cleavage of insulin-like growth factor binding proteins (IGFBPs) causing the release of IGFs. The reduced level of PAPP-A in smokers in first-trimester has been reported previously. However, smoking influence on maternal level of PAPP-A during pregnancy has not been completely determined. The aim of this preliminary study was the investigation of PAPP-A concentrations in smoking women in the first- and third-trimester of pregnancy. The study comprised 40 pregnant women: 20 nonsmokers and 20 cigarette smokers. Only normal singleton pregnancies were included. Maternal serum levels of PAPP-A were measured with the KRYPTOR rapid random-access immunoassay analyser (Brahms GmbH, Germany) using time-resolved amplified cryptate emission technology (TRACE). In first-trimester of pregnancy we observed the lower of PAPP-A median values in smokers (2.77 IU/L, range 1.12-9.28 IU/L) by 24% than in nonsmokers (3.64 IU/L, range 1.68-6.79 IU/L). Next, we indicated that in third-trimester of pregnancy the PAPP-A median values were lower in smokers (48.45 IU/L, range 15.18-176.2 IU/L) until by 50% in comparison to the controls (96.56 IU/L, range 25.59-164.0 IU/L). Further studies will be continued to assessment the influence of some components from tobacco smoke on maternal serum PAPP-A in all trimesters of pregnancy.
通过结合超声检查和生化标志物，孕早期染色体缺陷的产前筛查已得到改善。在这些标志物中，存在金属蛋白酶妊娠相关血浆蛋白A（PAPP-A），它通过蛋白水解切割胰岛素样生长因子结合蛋白（IGFBPs）促进细胞生长，从而导致胰岛素样生长因子（IGFs）的释放。先前已有报道称孕早期吸烟者体内PAPP-A水平降低。然而，吸烟对孕期母体PAPP-A水平的影响尚未完全确定。这项初步研究的目的是调查孕期头三个月和第三个月吸烟女性的PAPP-A浓度。该研究包括40名孕妇：20名非吸烟者和20名吸烟者。仅纳入正常单胎妊娠。使用时间分辨放大镧系元素荧光免疫分析技术（TRACE），通过KRYPTOR快速随机接入免疫分析仪（德国勃林格殷格翰公司）测量母体血清PAPP-A水平。在孕早期，我们观察到吸烟者的PAPP-A中位数（2.77 IU/L，范围1.12 - 9.28 IU/L）比非吸烟者（3.64 IU/L，范围1.68 - 6.79 IU/L）低24%。接下来，我们指出在孕晚期，吸烟者的PAPP-A中位数（48.45 IU/L，范围15.18 - 176.2 IU/L）比对照组（96.56 IU/L，范围25.59 - 164.0 IU/L）低50%。将继续进行进一步研究，以评估烟草烟雾中的某些成分对孕期各阶段母体血清PAPP-A的影响。
