Litvinova L S, Riazantseva N V, Novitskiĭ V V, Grigor'eva E S
Tsitologiia. 2008;50(1):67-71.
The influence of recombinant forms of cytokincs (IL-5, IL-3 and eotaxin) on programmed destruction of eosinophils obtained from patients with diseases associated with high eosinophilia of blood (malignant diseases of system of blood, opisthorchosis) was studied in vitro. It was shown that all examined categories of patients irrespective of their nosology demonstrated a low level of spontaneous apoptosis of eosinophils. Cultivation of cosinophils isolated from peripheral blood of the patients with invasion O. felineus in vitro with r-IL-5, r-IL-3 and r-eotaxin decreased the number of eosinophilic cells undergoing apoptosis in comparison with spontaneous one. At the same time, incubation of eosinophils obtained from the patients with malignant diseases of system of blood, associated with cosinophilic syndrome, with r-IL-5, r-IL-3 and r-eotaxin allowed to ascertain the absence of sensitivity of eosinophilic cells to the antiapoptotic effect of these cytocines.
在体外研究了重组细胞因子形式（IL-5、IL-3和嗜酸性粒细胞趋化因子）对从患有血液嗜酸性粒细胞增多相关疾病（血液系统恶性疾病、后睾吸虫病）患者获取的嗜酸性粒细胞程序性破坏的影响。结果显示，所有检查的患者类别，无论其疾病分类如何，嗜酸性粒细胞的自发凋亡水平均较低。用r-IL-5、r-IL-3和r-嗜酸性粒细胞趋化因子在体外培养从感染猫后睾吸虫患者外周血分离的嗜酸性粒细胞，与自发凋亡相比，经历凋亡的嗜酸性细胞数量减少。同时，用r-IL-5、r-IL-3和r-嗜酸性粒细胞趋化因子培养从患有与嗜酸性粒细胞综合征相关的血液系统恶性疾病患者获取的嗜酸性粒细胞，发现嗜酸性细胞对这些细胞因子的抗凋亡作用不敏感。
