Vu Kh Ch, Ondar U N, Soldatova O P
Ontogenez. 2008 Jan-Feb;39(1):8-14.
We have studied the morphology and vein branching of rosette leaves in Arabidopsis thaliana mutants as and sa, which proved to be alleles of the A. thaliana AS1 and AS2 genes, respectively. We have also analyzed the localization of bioactive auxin, as measured by the expression of the DR5::GUS transgene, as well as the expression patterns of BP, as measured by the expression of the BP::GUS transgene in leaves of the mutants. In mature leaves of the mutants, BP was expressed ectopically. Furthermore, the mutants showed some defects in the localization and concentration of free auxin compared to the wild type. Our results of studying new alleles of AS1 and AS2 support their role in control of class I KNOX genes and auxin transport.
我们研究了拟南芥突变体as和sa中莲座叶的形态和叶脉分支情况，结果证明它们分别是拟南芥AS1和AS2基因的等位基因。我们还分析了通过DR5::GUS转基因表达来衡量的生物活性生长素的定位，以及通过BP::GUS转基因在突变体叶片中的表达来衡量的BP表达模式。在突变体的成熟叶片中，BP异位表达。此外，与野生型相比，突变体在游离生长素的定位和浓度方面表现出一些缺陷。我们对AS1和AS2新等位基因的研究结果支持了它们在控制I类KNOX基因和生长素运输中的作用。
