Vasil'eva S V, Moshkovskaia E Iu, Terekhov A S, Mikoian V D, Vanin A F
Genetika. 2008 Jan;44(1):29-35.
In 2005 we have described in exponentially growing E. coli cells a new fundamental genetic phenomenon,--quasi-adaptive response to alkylating compounds (quasi-Ada). Phenotypic expression of quasi-Ada is similar to the true Ada response. However, in contrast to the letter, it develops in the course of pretreatment of the cells by a sublethal dose of nonalkylating agent, an NO-containing dinitrosyl iron complex with glutathione (DNICglu). To reveal the mechanisms of quasi-adaptation and its association with the function of the Ada regulatory protein, here we used a unique property of dual gene expression regulation of aidB1 gene, a part of the Ada-regulon, namely its relative independence from Ada protein in anaerobic conditions. Based on the results of aidB1 gene expression analysis an EPR spectra of E. coli MV2176 cells (aidB1::lacZ) in aerobic and anaerobic conditions after the corresponding treatments, we conclude that the function and the spatial structure of meAda and [(Cys-)2Fe+(NO+)2]Ada are identical and thus the nitrosylated protein represents a regulator of the Ada regulon gene expression during quasi-adaptation development.
2005年，我们在指数生长的大肠杆菌细胞中描述了一种新的基本遗传现象——对烷基化化合物的准适应性反应（quasi-Ada）。准Ada的表型表达与真正的Ada反应相似。然而，与后者不同的是，它是在细胞用亚致死剂量的非烷基化剂（一种含NO的与谷胱甘肽形成的二亚硝基铁络合物，DNICglu）预处理过程中产生的。为了揭示准适应的机制及其与Ada调节蛋白功能的关联，我们利用了Ada调节子一部分的aidB1基因的双基因表达调控的独特特性，即在厌氧条件下它相对独立于Ada蛋白。基于对相应处理后需氧和厌氧条件下大肠杆菌MV2176细胞（aidB1::lacZ）的aidB1基因表达分析及电子顺磁共振光谱结果，我们得出结论，meAda和[(Cys-)2Fe+(NO+)2]Ada的功能和空间结构相同，因此亚硝基化蛋白是准适应发展过程中Ada调节子基因表达的调节因子。
