Kugimiya Akimitsu, Morii Miki, Ohtsuki Takashi
Research Center for Advanced Science and Technology, The City of Hiroshima, Naka-ku, Hiroshima 730-0052, Japan.
Anal Biochem. 2008 Jul 1;378(1):90-2. doi: 10.1016/j.ab.2008.03.037. Epub 2008 Mar 25.
The detection of amino acids using aminoacyl-tRNA synthetases (ARSs) as the molecular recognition element was proposed, and the binding activity and specificity of ARSs were evaluated. Using this rapid and easy method, from 15 to 50 microM tyrosine could be measured specifically. The method suggested in this article could be realized without an amino acid labeling process or a large volume of organic solvents, and the time for measurement was reasonable.
提出了使用氨酰-tRNA合成酶（ARSs）作为分子识别元件来检测氨基酸，并评估了ARSs的结合活性和特异性。使用这种快速简便的方法，可以特异性地检测出15至50微摩尔的酪氨酸。本文所建议的方法无需氨基酸标记过程或大量有机溶剂即可实现，且测量时间合理。
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