Rab27a和JFC1作为前列腺癌细胞中前列腺特异性抗原分泌机制组成成分的特性研究
Characterization of Rab27a and JFC1 as constituents of the secretory machinery of prostate-specific antigen in prostate carcinoma cells.
作者信息
Catz Sergio D
机构信息
Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA.
出版信息
Methods Enzymol. 2008;438:25-40. doi: 10.1016/S0076-6879(07)38003-8.
Abstract
Prostate-specific antigen (PSA) and prostate-specific acid phosphatase (PSAP) are produced by prostate carcinoma cells. Their secretion has implications in both prostate cancer diagnosis and progression. The mechanisms involved in PSA and PSAP secretion in response to androgens have remained relatively unknown. The small GTPase Rab27a regulates exocytosis in several tissues. Here, we present methods for the characterization of Rab27a and its effector JFC1/Slp1 as key components of the secretory machinery that regulates exocytosis in prostate carcinoma cells.
摘要
前列腺特异性抗原（PSA）和前列腺特异性酸性磷酸酶（PSAP）由前列腺癌细胞产生。它们的分泌对前列腺癌的诊断和进展均有影响。雄激素刺激下PSA和PSAP分泌所涉及的机制仍相对不明。小GTP酶Rab27a在多个组织中调节胞吐作用。在此，我们介绍了一些方法，用于将Rab27a及其效应器JFC1/Slp1鉴定为调节前列腺癌细胞胞吐作用的分泌机制的关键组成部分。
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