[Optimization of cultivation conditions of the alpha-amylase producer Bacillus subtilis 147].

The influence of some technological parameters of cultivation of the producer Bacillus subtilis 147 on the process of extracellular enzymes alpha-amylase synthesis was investigated. The optimum sources of carbon (0.1% insoluble starch) and nitrogen (0.2% sodium nitrate) for maximum production of alpha-amylase are established. It was shown, that the temperature 42 degrees C, the carbon nitrogen ratio 1:2, pH 7.0, volume of a nutritious medium 100 ml, rotation rate 220 rev/min during 4-6 days are optimum parameters of the producer cultivation. As a result the enzyme activity was increased 14 times.