[分批和连续条件下α-淀粉酶产生菌枯草芽孢杆菌的培养]
[Cultivation of the producer of alpha-amylase Bacillus subtilis under batch and continuous conditions].
作者信息
Lirova S A, Ermakova L M, Rabotnova I L, Khovrychev M P
出版信息
Mikrobiologiia. 1988 Sep-Oct;57(5):740-4.
The growth of Bacillus subtilis FU-79 and its production of alpha-amylase (EC 3.2.1.1) were studied under the conditions of batch and continuous cultivation in a semisynthetic medium. The enzyme activity fell down abruptly upon a pulse addition of either glucose or yeast extract to the chemostat culture, and remained at a low level for the following ten generations. Apparently, a double limitation of the culture growth (viz., with residual glucose and with yeast extract components) is required for the activity of alpha-amylase to be high.
在半合成培养基中分批培养和连续培养的条件下，研究了枯草芽孢杆菌FU-79的生长及其α-淀粉酶（EC 3.2.1.1）的产生。向恒化器培养物中脉冲添加葡萄糖或酵母提取物后，酶活性会突然下降，并在接下来的十代中保持在较低水平。显然，α-淀粉酶活性要高需要培养物生长的双重限制（即，同时存在残留葡萄糖和酵母提取物成分）。
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