温和扩展的酶促糖基多样化反应

Sweetly expanding enzymatic glycodiversification.

作者信息

Jakeman David L

机构信息

College of Pharmacy and Department of Chemistry, Dalhousie University, Halifax, Nova Scotia B3H 3J5, Canada.

出版信息

Chem Biol. 2008 Apr;15(4):307-8. doi: 10.1016/j.chembiol.2008.03.009.

DOI:10.1016/j.chembiol.2008.03.009

PMID:18420135

Abstract

Directed evolution is a powerful tool to modify substrate specificity for a wide array of enzyme catalysts. In this issue of Chemistry & Biology, Thorson and coworkers use directed evolution to increase the catalytic proficiency of a model glycosyltransferase, OleD, 300-fold for a nonphysiological substrate (Williams et al., 2008).

摘要

定向进化是一种强大的工具，可用于改变多种酶催化剂的底物特异性。在本期《化学生物学》中，索尔森及其同事利用定向进化将一种模型糖基转移酶OleD对非生理性底物的催化效率提高了300倍（威廉姆斯等人，2008年）。