嗜碱假单胞菌KF707和恶臭假单胞菌KF715中多氯联苯（PCB）降解菌的高度保守编码序列。

Highly conserved coding sequences in polychlorinated biphenyl (PCB)-degraders of Pseudomonas pseudoalcaligenes KF707 and Pseudomonas putida KF715.

作者信息

Furukawa K, Nakagawa K, Yuyama N, Sato A, Kokean Y, Kato H, Yamashita S, Hayase N, Nishikawa S, Taira K

机构信息

Fermentation Research Institute, Agency of Industrial Science & Technology, MITI, Tsukuba Science City, Japan.

出版信息

Nucleic Acids Symp Ser. 1991(25):115-6.

PMID:1842046

Abstract

Sixteen strains that utilize biphenyl or polychlorinated biphenyl (PCB) as the sole source of carbon and energy have previously been isolated. Nucleotide sequence of the bphABCXD operon (11 kb) of Pseudomonas pseudoalcaligenes KF707 has now been determined. When bphD region is compared with the previously reported bphD sequence of another PCB-degrader, Pseudomonas putida KF715, homologies at the level of nucleotides as well as amino acids are as high as 96%. Moreover, both bphABCXD operon (KF707) and bphABCD operon (KF715) share the identical transcriptional initiation site. Since these aromatic hydrocarbon-degraders are coded on chromosomes, whereas other majorities of hydrocarbon-degraders are coded on plasmids, there must be a mechanism governing the chromosomal gene-shuffling.

摘要

此前已分离出16种以联苯或多氯联苯（PCB）作为唯一碳源和能源的菌株。现已确定了假产碱假单胞菌KF707的bphABCXD操纵子（11 kb）的核苷酸序列。当将bphD区域与先前报道的另一种多氯联苯降解菌恶臭假单胞菌KF715的bphD序列进行比较时，核苷酸水平和氨基酸水平的同源性高达96%。此外，bphABCXD操纵子（KF707）和bphABCD操纵子（KF715）具有相同的转录起始位点。由于这些芳烃降解菌的基因编码在染色体上，而其他大多数烃降解菌的基因编码在质粒上，因此必定存在一种控制染色体基因重排的机制。