Cheng Jinquan, Liu Jianping, Zhang Renli, Yu Lei
Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.
Wei Sheng Yan Jiu. 2008 Jan;37(1):1-3, 7.
To explore the relationship between A-922G genetic polymorphism in the 5' flanking region of the endothelial nitric oxide synthase (eNOS) gene and ischemic stroke.
A 1:1 matched case-control study was conducted. 309 patients suffered from ischemic stroke were selected from two general hospitals in Shenzhen. The controls were selected by the same gender and ethnic group, and each pair's ages were permitted of differ within 5 years. eNOS A-922G genotypes were determined by using the real-time PCR technology for SNP genotyping with Taqman MGB probes. The cases and controls were interviewed by using the same questionnaire. Results The genotype frequencies (AA, AG and GG) of eNOS A-922G were 78.96%, 17.80, and 3.24% in patients, and 85.11%, 13.59, and 1.29% in controls. The allele frequencies of eNOS--922G in case group (12.14%) were higher than those in the controls (8.09%) (P = 0.018). OR value of ischemic stroke for subjects with at least one G allele was 1.523 (OR 95% CI 1.005-2.309). Results of conditional logistic regression demonstrated that eNOS A-922G genetic polymorphism also had significant effects on ischemic stroke (OR = 2.156, 95% CI 1.081-4.299) after adjusting smoking, alcohol drinking, waist-to-hipratio, body mass index and history of hypertension, et al.
It was suggested that A-922G gene polymorphism of eNOS gene could associate with the higher risk of ischemic stroke susceptibility.
探讨内皮型一氧化氮合酶（eNOS）基因5′侧翼区A-922G基因多态性与缺血性脑卒中的关系。
进行1∶1匹配的病例对照研究。从深圳两家综合医院选取309例缺血性脑卒中患者。对照组按性别和种族匹配选取，每对年龄相差不超过5岁。采用Taqman MGB探针实时荧光定量PCR技术进行SNP基因分型，检测eNOS A-922G基因型。采用相同问卷对病例组和对照组进行调查。结果缺血性脑卒中患者eNOS A-922G基因型频率（AA、AG和GG）分别为78.96%、17.80%和3.24%，对照组分别为85.11%、13.59%和1.29%。病例组eNOS -922G等位基因频率（12.14%）高于对照组（8.09%）（P = 0.018）。至少携带一个G等位基因的受试者发生缺血性脑卒中的OR值为1.523（OR 95%CI 1.005 -
2.309）。条件logistic回归分析结果显示，在调整吸烟、饮酒、腰臀比、体重指数和高血压病史等因素后，eNOS A-922G基因多态性对缺血性脑卒中仍有显著影响（OR = 2.156，95%CI 1.081 - 4.299）。
提示eNOS基因A-922G基因多态性可能与缺血性脑卒中易感性的较高风险相关。
