Dai Shou-feng, Shen Qi, Chen Jing, Chen Shu-qing
Institution of Pharmacology & Toxicology and Biological Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2008 Mar;37(2):134-8. doi: 10.3785/j.issn.1008-9292.2008.02.005.
To investigate the causes and influencing factors of heterogeneity of HSA/IL1ra fusion protein expression in Pichia pastoris.
The heterogeneity of HSA/IL1ra fusion protein expressed in Pichia pastoris was studied by removing glycosylation and inhibiting glycosylation, as well as by different ways of fusion, different clones, and different expression host.
Glycosylation caused expression heterogeneity of fusion protein, but in SMD1168 and some GS115 clones there was no this phenomenon.
The expression heterogeneity of HSA/IL1ra fusion protein in Pichia pastoris is due to the glycosylation, and different ways of fusion, different clones, different expression host also have some impact.
研究毕赤酵母中HSA/IL1ra融合蛋白表达异质性的原因及影响因素。
通过去除糖基化、抑制糖基化以及不同的融合方式、不同的克隆和不同的表达宿主，研究毕赤酵母中表达的HSA/IL1ra融合蛋白的异质性。
糖基化导致融合蛋白表达异质性，但在SMD1168和一些GS115克隆中不存在这种现象。
毕赤酵母中HSA/IL1ra融合蛋白的表达异质性是由于糖基化，不同的融合方式、不同的克隆、不同的表达宿主也有一定影响。
