Pieri Francesco, Lucarelli Enrico, Corinaldesi Giuseppe, Iezzi Giovanna, Piattelli Adriano, Giardino Roberto, Bassi Massimo, Donati Davide, Marchetti Claudio
Department of Odontostomatological Sciences, University of Bologna, Bologna, Italy.
J Clin Periodontol. 2008 Jun;35(6):539-46. doi: 10.1111/j.1600-051X.2008.01220.x. Epub 2008 Apr 15.
Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue-engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone-to-implant contact (BIC) in maxillary sinus grafting.
Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis.
After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51%versus 18.98%; p=0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71%versus 6.63%; p=0.028).
These findings show that sinus augmentation with MSCs-PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.
用于上颌窦提升的自体、异体和异种材料都有特定的缺点，这激发了人们对新材料和组织工程构建物的持续探索。我们研究了接种在氟羟基磷灰石（FH）支架上的间充质干细胞（MSCs）和富血小板血浆（PRP）是否能改善上颌窦植骨中的骨形成和骨与种植体的接触（BIC）。
对8只小型猪进行双侧上颌窦提升手术。在每个上颌窦中植入MSCs、PRP和FH支架（试验部位）或仅植入FH（对照部位）。在截骨远端，通过面部窦壁在移植材料中每个窦植入一枚牙种植体。移植后3个月处死动物，采集种植部位的块状切片并准备进行组织形态计量分析。
12周后，与对照部位相比，试验部位的骨形成显著增加（42.51%对18.98%；p = 0.001）。此外，在再生区域，试验部位的BIC显著高于对照部位（23.71%对6.63%；p = 0.028）。
这些研究结果表明，在小型猪中，与单独使用FH相比，用MSCs - PRP联合FH进行上颌窦提升可能会增强牙种植体的骨形成和骨整合。
