Construction of a reporter vector system for in vivo analysis of promoter activity in Propionibacterium freudenreichii.
作者信息
Faye Therese, Asebø Anita, Salehian Zhian, Langsrud Thor, Nes Ingolf F, Brede Dag Anders
机构信息
Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (UMB), PO Box 5003, N-1432 As, Norway.
出版信息
Appl Environ Microbiol. 2008 Jun;74(11):3615-7. doi: 10.1128/AEM.02870-07. Epub 2008 Apr 18.
Abstract
A beta-galactosidase reporter system for the analysis of promoter elements in Propionibacterium freudenreichii was designed. The pTD210 in vivo reporter vector was constructed using a promoterless lacZ gene from Bifidobacterium longum cloned into the pAMT1 plasmid. The utility of the pTD210 reporter vector was demonstrated by an investigation of six predicted promoters in P. freudenreichii. The system produced accurate and reproducible measurements that facilitated both promoter identification and the quantification of promoter activities.
摘要
相似文献
1
Construction of a reporter vector system for in vivo analysis of promoter activity in Propionibacterium freudenreichii.
Appl Environ Microbiol. 2008 Jun;74(11):3615-7. doi: 10.1128/AEM.02870-07. Epub 2008 Apr 18.
2
Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
Appl Environ Microbiol. 2006 Nov;72(11):7401-5. doi: 10.1128/AEM.01611-06. Epub 2006 Sep 22.
3
Construction of targeted and integrative promoter-reporter plasmids pDK-K and pDK-G to measure gene expression activity in Haemophilus parasuis.
Microb Pathog. 2019 Sep;134:103565. doi: 10.1016/j.micpath.2019.103565. Epub 2019 May 31.
4
Heterologous production of antimicrobial peptides in Propionibacterium freudenreichii.
Appl Environ Microbiol. 2005 Dec;71(12):8077-84. doi: 10.1128/AEM.71.12.8077-8084.2005.
5
Construction of an expression vector for propionibacteria and its use in production of 5-aminolevulinic acid by Propionibacterium freudenreichii.
Appl Microbiol Biotechnol. 2001 Jul;56(1-2):144-9. doi: 10.1007/s002530100603.
6
Construction of a versatile promoter analysis vector and its use for analysis of the Serratia marcescens aspartase promoter region.
Plasmid. 1994 Sep;32(2):233-7. doi: 10.1006/plas.1994.1061.
7
Characterization of pRGO1, a plasmid from Propionibacterium acidipropionici, and its use for development of a host-vector system in propionibacteria.
Appl Environ Microbiol. 2000 Nov;66(11):4688-95. doi: 10.1128/AEM.66.11.4688-4695.2000.
8
Identification of the propionicin F bacteriocin immunity gene (pcfI) and development of a food-grade cloning system for Propionibacterium freudenreichii.
Appl Environ Microbiol. 2007 Dec;73(23):7542-7. doi: 10.1128/AEM.01023-07. Epub 2007 Oct 12.
9
Construction of lacZ promoter probe vectors for use in Synechococcus: application to the identification of CO2-regulated promoters.
Gene. 1990 May 31;90(1):43-9. doi: 10.1016/0378-1119(90)90437-v.
10
Transcription reporters that shuttle cloned DNA between high-copy Escherichia coli plasmids and low-copy broad-host-range plasmids.
Plasmid. 2000 Sep;44(2):152-62. doi: 10.1006/plas.2000.1471.
引用本文的文献
1
A lacZ Reporter-Based Strategy for Rapid Expression Analysis and Target Validation of Mycobacterium tuberculosis Latent Infection Genes.
Curr Microbiol. 2016 Feb;72(2):213-219. doi: 10.1007/s00284-015-0942-3. Epub 2015 Nov 23.
本文引用的文献
1
Identification of the propionicin F bacteriocin immunity gene (pcfI) and development of a food-grade cloning system for Propionibacterium freudenreichii.
Appl Environ Microbiol. 2007 Dec;73(23):7542-7. doi: 10.1128/AEM.01023-07. Epub 2007 Oct 12.
2
The Genomes On Line Database (GOLD) v.2: a monitor of genome projects worldwide.
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D332-4. doi: 10.1093/nar/gkj145.
3
Heterologous production of antimicrobial peptides in Propionibacterium freudenreichii.
Appl Environ Microbiol. 2005 Dec;71(12):8077-84. doi: 10.1128/AEM.71.12.8077-8084.2005.
4
Molecular analysis of promoter elements from Propionibacterium freudenreichii.
J Biosci Bioeng. 2004;97(5):310-6. doi: 10.1016/S1389-1723(04)70211-X.
5
Molecular and genetic characterization of propionicin F, a bacteriocin from Propionibacterium freudenreichii.
Appl Environ Microbiol. 2004 Dec;70(12):7303-10. doi: 10.1128/AEM.70.12.7303-7310.2004.
6
The complete genome sequence of Propionibacterium acnes, a commensal of human skin.
Science. 2004 Jul 30;305(5684):671-3. doi: 10.1126/science.1100330.
7
Construction of an expression vector for propionibacteria and its use in production of 5-aminolevulinic acid by Propionibacterium freudenreichii.
Appl Microbiol Biotechnol. 2001 Jul;56(1-2):144-9. doi: 10.1007/s002530100603.
8
Biochemical and genetic characterization of propionicin T1, a new bacteriocin from Propionibacterium thoenii.
Appl Environ Microbiol. 2000 Oct;66(10):4230-6. doi: 10.1128/AEM.66.10.4230-4236.2000.
9
Nucleotide sequence, expression and transcriptional analysis of the Bifidobacterium longum MB 219 lacZ gene.
Arch Microbiol. 2000 Jul-Aug;174(1-2):74-80. doi: 10.1007/s002030000178.
10
Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues.
Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8540-4. doi: 10.1073/pnas.96.15.8540.
Zotero 插件