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2
Sequence analysis of two cryptic plasmids from Bifidobacterium longum DJO10A and construction of a shuttle cloning vector.长双歧杆菌DJO10A两个隐蔽质粒的序列分析及穿梭克隆载体的构建
Appl Environ Microbiol. 2006 Jan;72(1):527-35. doi: 10.1128/AEM.72.1.527-535.2006.
3
The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR.质粒和其他遗传元件的拷贝数可以通过基于SYBR Green的定量实时PCR来确定。
J Microbiol Methods. 2006 Jun;65(3):476-87. doi: 10.1016/j.mimet.2005.09.007. Epub 2005 Oct 10.
4
Lessons from the genomes of bifidobacteria.双歧杆菌基因组的启示。
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Structural and functional analysis of pTB6 from Bifidobacterium longum.长双歧杆菌中pTB6的结构与功能分析
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Species specific identification of nine human Bifidobacterium spp. in feces.粪便中九种人类双歧杆菌属物种的特异性鉴定。
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Sequence analysis of plasmid pKJ50 from Bifidobacterium longum.长双歧杆菌质粒pKJ50的序列分析
Microbiology (Reading). 1999 Mar;145 ( Pt 3):585-592. doi: 10.1099/13500872-145-3-585.
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Construction of Escherichia coli-Bifidobacterium longum shuttle vector transforming B. longum 105-A and 108-A.构建转化长双歧杆菌105 - A和108 - A的大肠杆菌-长双歧杆菌穿梭载体。
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用于分析长双歧杆菌启动子的报告载体构建

Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.

作者信息

Klijn A, Moine D, Delley M, Mercenier A, Arigoni F, Pridmore R D

机构信息

Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.

出版信息

Appl Environ Microbiol. 2006 Nov;72(11):7401-5. doi: 10.1128/AEM.01611-06. Epub 2006 Sep 22.

DOI:10.1128/AEM.01611-06
PMID:16997985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1636199/
Abstract

In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.

摘要

为了启动对长双歧杆菌启动子活性的研究，并独立确认微阵列实验产生的转录数据，我们基于长双歧杆菌的一个隐蔽质粒和大肠杆菌的gusA基因构建了一个通用报告质粒。所得质粒pMDY23已用三个长双歧杆菌启动子进行了测试。