用于分析长双歧杆菌启动子的报告载体构建
Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.
作者信息
Klijn A, Moine D, Delley M, Mercenier A, Arigoni F, Pridmore R D
机构信息
Department of Nutrition and Health, Nestlé Research Center, P.O. Box 44, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland.
出版信息
Appl Environ Microbiol. 2006 Nov;72(11):7401-5. doi: 10.1128/AEM.01611-06. Epub 2006 Sep 22.
Abstract
In order to initiate studies on promoter activities in Bifidobacterium longum and to independently confirm transcriptional data generated by microarray experiments, we have constructed a versatile reporter plasmid based on a B. longum cryptic plasmid and the Escherichia coli gusA gene. The resulting plasmid, pMDY23, has been tested using three B. longum promoters.
摘要
为了启动对长双歧杆菌启动子活性的研究，并独立确认微阵列实验产生的转录数据，我们基于长双歧杆菌的一个隐蔽质粒和大肠杆菌的gusA基因构建了一个通用报告质粒。所得质粒pMDY23已用三个长双歧杆菌启动子进行了测试。
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