Tan Eng Lee, Marcus Kah Fai Ho, Poh Chit Laa
School of Chemical and Life Sciences, Singapore Polytechnic, Singapore, Singapore.
J Med Virol. 2008 Jun;80(6):1025-32. doi: 10.1002/jmv.21210.
Enterovirus 70 (EV70) is recognized as the main causative agent of acute hemorrhagic conjunctivitis (AHC), a highly contagious viral infection of the eye. Currently, there is no available treatment for EV70 infections. In this study, we developed a potential intervention strategy using RNA interference (RNAi) against EV70 infection in an in vitro system. Two synthetic 19-mer siRNAs, si-3D1 and si-3D2, were designed to target the 3Dpol region of the EV70 genome. Significant dosage dependent inhibition of EV70 in rhabdomyosarcoma cell line, as shown by reduction of viral RNA and VP1 production, was observed. Both siRNAs prevented EV70 replication in RD cells when transfected into these cells 48 hr prior to virus infection. Introduction of these siRNAs into RD cells 1-3 hr after infection with EV70 reduced production of viral RNA by approximately 60%. Thus, RNAi is a promising strategy to prevent EV70 infections and may have therapeutic potential.
肠道病毒70型（EV70）被认为是急性出血性结膜炎（AHC）的主要病原体，AHC是一种眼部高度传染性病毒感染。目前，尚无针对EV70感染的有效治疗方法。在本研究中，我们在体外系统中开发了一种利用RNA干扰（RNAi）对抗EV70感染的潜在干预策略。设计了两种合成的19聚体小干扰RNA（siRNA），即si-3D1和si-3D2，以靶向EV70基因组的3D聚合酶区域。观察到在横纹肌肉瘤细胞系中，EV70受到显著的剂量依赖性抑制，表现为病毒RNA和VP1产量的降低。当在病毒感染前48小时将这两种siRNA转染到RD细胞中时，它们都能阻止EV70在RD细胞中的复制。在感染EV70后1至3小时将这些siRNA导入RD细胞，可使病毒RNA产量降低约60%。因此，RNAi是预防EV70感染的一种有前景的策略，可能具有治疗潜力。
